Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis

Bagatella, S.; Haghayegh Jahromi, N.; Monney, C.; Polidori, M.; Gall, F.M.; Serra, F.; Riedl, R.; Engelhardt, B.; Oevermann, A.

doi:10.1016/j.jcpa.2023.03.073

Publication type:	Conference other
Type of review:	Peer review (abstract)
Title:	Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis
Authors:	Bagatella, S. Haghayegh Jahromi, N. Monney, C. Polidori, M. Gall, F.M. Serra, F. Riedl, R. Engelhardt, B. Oevermann, A.
et. al:	No
DOI:	10.1016/j.jcpa.2023.03.073
Published in:	Journal of Comparative Pathology
Volume(Issue):	203
Page(s):	61
Conference details:	Annual Congress of the European Society of Veterinary Pathology and European College of Veterinary Pathologists, Athens, Greece, September 7‒10, 2022
Issue Date:	8-Oct-2022
Publisher / Ed. Institution:	Elsevier
ISSN:	0021-9975 1532-3129
Language:	English
Subject (DDC):	572: Biochemistry 579: Microbiology
Abstract:	Introduction: The bacterium Listeria monocytogenes (LM) can cause life-threatening central nervous system (CNS) infection (neurolisteriosis) in humans and ruminants. Polymorphonuclear neutrophils (PMNs) recruited into the CNS migrate to infectious foci to exert bacterial killing, but overzealous PMN recruitment is regularly linked to serious neural damage. Factors responsible for PMN chemotaxis to CNS infectious foci in neurolisteriosis remain obscure. Here, we aimed at identifying chemotactic factors (bacteria vs host-derived) for bovine PMNs in neurolisteriosis. Materials and methods: We performed ex-vivo chemotaxis assays with primary bovine PMNs to assess the chemotactic effect of LM components and supernatants of endogenous CNS cells infected with LM. IL-8 (main chemotactic candidate) expression was also assessed through ELISA and in-situ immunohistochemistry/immunofluorescence in CNS of naturally infected cattle. Results: Whole bacteria and LM cell wall components failed to elicit PMN chemotaxis, as did supernatants from LM-infected astrocytes and neural cells. Conversely, supernatant of LM-infected microglia proved chemotactic for PMNs. Moreover, microglial secretion of IL-8 was detected on in-vitro microglial infection and in microglia of cattle with neurolisteriosis in situ. Conclusions: Our results indicate that PMN chemotaxis to infectious foci in neurolisteriosis is independent of bacterial stimuli and is rather mediated by host-derived chemotactic factors (including IL-8) produced by microglia. Prevention of excessive PMN recruitment by blocking such factors may constitute a candidate therapeutic option to limit PMN-mediated CNS damage in neurolisteriosis.
URI:	https://digitalcollection.zhaw.ch/handle/11475/30071
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Bagatella, S., Haghayegh Jahromi, N., Monney, C., Polidori, M., Gall, F. M., Serra, F., Riedl, R., Engelhardt, B., & Oevermann, A. (2022). Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis [Conference presentation]. Journal of Comparative Pathology, 203, 61. https://doi.org/10.1016/j.jcpa.2023.03.073

Bagatella, S. et al. (2022) ‘Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis’, in Journal of Comparative Pathology. Elsevier, p. 61. Available at: https://doi.org/10.1016/j.jcpa.2023.03.073.

S. Bagatella et al., “Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis,” in Journal of Comparative Pathology, Oct. 2022, vol. 203, p. 61. doi: 10.1016/j.jcpa.2023.03.073.

BAGATELLA, S., N. HAGHAYEGH JAHROMI, C. MONNEY, M. POLIDORI, F.M. GALL, F. SERRA, R. RIEDL, B. ENGELHARDT und A. OEVERMANN, 2022. Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis. In: Journal of Comparative Pathology. Conference presentation. Elsevier. 8 Oktober 2022. S. 61

Bagatella, S., N. Haghayegh Jahromi, C. Monney, M. Polidori, F.M. Gall, F. Serra, R. Riedl, B. Engelhardt, and A. Oevermann. 2022. “Microglia, rather than Bacterial Factors, Mediate Bovine Neutrophil Chemotaxis in Neurolisteriosis.” Conference presentation. In Journal of Comparative Pathology, 203:61. Elsevier. https://doi.org/10.1016/j.jcpa.2023.03.073.

Bagatella, S., et al. “Microglia, rather than Bacterial Factors, Mediate Bovine Neutrophil Chemotaxis in Neurolisteriosis.” Journal of Comparative Pathology, vol. 203, Elsevier, 2022, p. 61, https://doi.org/10.1016/j.jcpa.2023.03.073.