| Publikationstyp: | Konferenz: Sonstiges |
| Art der Begutachtung: | Peer review (Abstract) |
| Titel: | Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis |
| Autor/-in: | Bagatella, S. Haghayegh Jahromi, N. Monney, C. Polidori, M. Gall, F.M. Serra, F. Riedl, R. Engelhardt, B. Oevermann, A. |
| et. al: | No |
| DOI: | 10.1016/j.jcpa.2023.03.073 |
| Erschienen in: | Journal of Comparative Pathology |
| Band(Heft): | 203 |
| Seite(n): | 61 |
| Angaben zur Konferenz: | Annual Congress of the European Society of Veterinary Pathology and European College of Veterinary Pathologists, Athens, Greece, September 7‒10, 2022 |
| Erscheinungsdatum: | 8-Okt-2022 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 0021-9975 1532-3129 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 572: Biochemie 579: Mikrobiologie |
| Zusammenfassung: | Introduction: The bacterium Listeria monocytogenes (LM) can cause life-threatening central nervous system (CNS) infection (neurolisteriosis) in humans and ruminants. Polymorphonuclear neutrophils (PMNs) recruited into the CNS migrate to infectious foci to exert bacterial killing, but overzealous PMN recruitment is regularly linked to serious neural damage. Factors responsible for PMN chemotaxis to CNS infectious foci in neurolisteriosis remain obscure. Here, we aimed at identifying chemotactic factors (bacteria vs host-derived) for bovine PMNs in neurolisteriosis. Materials and methods: We performed ex-vivo chemotaxis assays with primary bovine PMNs to assess the chemotactic effect of LM components and supernatants of endogenous CNS cells infected with LM. IL-8 (main chemotactic candidate) expression was also assessed through ELISA and in-situ immunohistochemistry/immunofluorescence in CNS of naturally infected cattle. Results: Whole bacteria and LM cell wall components failed to elicit PMN chemotaxis, as did supernatants from LM-infected astrocytes and neural cells. Conversely, supernatant of LM-infected microglia proved chemotactic for PMNs. Moreover, microglial secretion of IL-8 was detected on in-vitro microglial infection and in microglia of cattle with neurolisteriosis in situ. Conclusions: Our results indicate that PMN chemotaxis to infectious foci in neurolisteriosis is independent of bacterial stimuli and is rather mediated by host-derived chemotactic factors (including IL-8) produced by microglia. Prevention of excessive PMN recruitment by blocking such factors may constitute a candidate therapeutic option to limit PMN-mediated CNS damage in neurolisteriosis. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/30071 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Bagatella, S., Haghayegh Jahromi, N., Monney, C., Polidori, M., Gall, F. M., Serra, F., Riedl, R., Engelhardt, B., & Oevermann, A. (2022). Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis [Conference presentation]. Journal of Comparative Pathology, 203, 61. https://doi.org/10.1016/j.jcpa.2023.03.073
Bagatella, S. et al. (2022) ‘Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis’, in Journal of Comparative Pathology. Elsevier, p. 61. Available at: https://doi.org/10.1016/j.jcpa.2023.03.073.
S. Bagatella et al., “Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis,” in Journal of Comparative Pathology, Oct. 2022, vol. 203, p. 61. doi: 10.1016/j.jcpa.2023.03.073.
BAGATELLA, S., N. HAGHAYEGH JAHROMI, C. MONNEY, M. POLIDORI, F.M. GALL, F. SERRA, R. RIEDL, B. ENGELHARDT und A. OEVERMANN, 2022. Microglia, rather than bacterial factors, mediate bovine neutrophil chemotaxis in neurolisteriosis. In: Journal of Comparative Pathology. Conference presentation. Elsevier. 8 Oktober 2022. S. 61
Bagatella, S., N. Haghayegh Jahromi, C. Monney, M. Polidori, F.M. Gall, F. Serra, R. Riedl, B. Engelhardt, and A. Oevermann. 2022. “Microglia, rather than Bacterial Factors, Mediate Bovine Neutrophil Chemotaxis in Neurolisteriosis.” Conference presentation. In Journal of Comparative Pathology, 203:61. Elsevier. https://doi.org/10.1016/j.jcpa.2023.03.073.
Bagatella, S., et al. “Microglia, rather than Bacterial Factors, Mediate Bovine Neutrophil Chemotaxis in Neurolisteriosis.” Journal of Comparative Pathology, vol. 203, Elsevier, 2022, p. 61, https://doi.org/10.1016/j.jcpa.2023.03.073.
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