Title: Simple and rapid sample preparation for 25OH-Vitamin D3/D2 using an immobilized liquid
Authors : Weibel, Roland
Zaborosch, Christiane
Svoboda, Michal
Geyer, Roland
Bartl, Ralf
Conference details: MSACL Mass Spectrometry Applications to the Clinical Lab, San Diego CA, 5-9 February 2011
Issue Date: Feb-2011
License (according to publishing contract) : Not specified
Type of review: No review
Language : English
Subjects : Diagnostic; Sample preparation; Mass spectrometry; Vitamin D
Subject (DDC) : 540: Chemistry
Abstract: The metabolites 25OH-Vitamin D3/D2 represent the storage form of Vitamin D for the human body. Healthy subjects should display concentrations between 30 - 70 ng/mL in plasma while a level below 30 ng/mL indicates Vitamin D deficiency. Monitoring 25OH-Vit D3/D2 e.g. by LC-MS/MS is crucial for the detection of those deficiencies which result in severe health threats if not treated in a timely manner. Prior to any analysis by LC-MS/MS, however, most of the matrix - which means proteins, lipids, carbohydrates, salts and others - has to be removed from biological samples like serum or plasma. Extraction using an immobilized liquid is a rapid and simple method to produce sample extracts that are sufficiently matrix-depleted to be directly injected onto an analytical column for quantification by LCMS/MS. In addition, this sample preparation procedure bears a promising potential for straight forward automation.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Conference Poster
URI: https://digitalcollection.zhaw.ch/handle/11475/9213
Appears in Collections:Publikationen Life Sciences und Facility Management

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