|Title:||Simple and rapid sample preparation for 25OH-Vitamin D3/D2 using an immobilized liquid|
|Authors :||Weibel, Roland|
|Conference details:||MSACL Mass Spectrometry Applications to the Clinical Lab, San Diego CA, 5-9 February 2011|
|License (according to publishing contract) :||Not specified|
|Type of review:||No review|
|Subjects :||Diagnostic; Sample preparation; Mass spectrometry; Vitamin D|
|Subject (DDC) :||540: Chemistry|
|Abstract:||The metabolites 25OH-Vitamin D3/D2 represent the storage form of Vitamin D for the human body. Healthy subjects should display concentrations between 30 - 70 ng/mL in plasma while a level below 30 ng/mL indicates Vitamin D deficiency. Monitoring 25OH-Vit D3/D2 e.g. by LC-MS/MS is crucial for the detection of those deficiencies which result in severe health threats if not treated in a timely manner. Prior to any analysis by LC-MS/MS, however, most of the matrix - which means proteins, lipids, carbohydrates, salts and others - has to be removed from biological samples like serum or plasma. Extraction using an immobilized liquid is a rapid and simple method to produce sample extracts that are sufficiently matrix-depleted to be directly injected onto an analytical column for quantification by LCMS/MS. In addition, this sample preparation procedure bears a promising potential for straight forward automation.|
|Departement:||Life Sciences und Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Publication type:||Conference Poster|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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