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dc.contributor.authorWeibel, Roland-
dc.contributor.authorZaborosch, Christiane-
dc.contributor.authorSvoboda, Michal-
dc.contributor.authorGeyer, Roland-
dc.contributor.authorBartl, Ralf-
dc.description.abstractThe metabolites 25OH-Vitamin D3/D2 represent the storage form of Vitamin D for the human body. Healthy subjects should display concentrations between 30-70 ng/mL in plasma while a level below 30 ng/mL indicates Vitamin D deficiency. Monitoring 25OH-Vit D3/D2 e.g. by LC-MS/MS is crucial for the detection of those deficiencies which result in severe health threats if not treated in a timely manner. Prior to any analysis by LC-MS/MS, however, most of the matrix – which means proteins, lipids, carbohydrates, salts and others – has to be removed from biological samples like serum or plasma. Extraction using an immobilized liquid is a rapid and simple method to produce sample extracts that are sufficiently matrix-depleted to be directly injected onto an analytical column for quantification by LCMS/MS. In addition, this sample preparation procedure bears a promising potential for straight forward automation.de_CH
dc.rightsNot specifiedde_CH
dc.subjectSample preparationde_CH
dc.subjectMass spectrometryde_CH
dc.subjectVitamin Dde_CH
dc.subject.ddc540: Chemiede_CH
dc.titleSimple and rapid sample preparation for 25OH-Vitamin D3/D2 using an immobilized liquidde_CH
dc.typeKonferenz: Posterde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.conference.detailsMSACL Mass Spectrometry Applications to the Clinical Lab, San Diego, USA, 5-9 February 2011de_CH
zhaw.publication.reviewKeine Begutachtungde_CH
Appears in Collections:Publikationen Life Sciences und Facility Management

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