Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures
Authors: Yildirim, Selçuk
Konrad, Daniel
Calvez, Ségolène
Drider, Djamel
Prévost, Hervé
Lacroix, Christophe
DOI: 10.1007/s00253-007-1188-1
Published in: Applied Microbiology and Biotechnology
Volume(Issue): 77
Issue: 3
Pages: 525
Pages to: 531
Issue Date: Dec-2007
Publisher / Ed. Institution: Springer
ISSN: 0175-7598
Language: English
Subject (DDC): 660.6: Biotechnology
Abstract: To increase the yield of heterologous production of the class II bacteriocin DvnRV41 with Escherichia coli Origami (DE3) (pLysS/pCR03), induction of bacteriocin gene expression was optimized by varying the inducer isopropyl β-d-thiogalactopyranoside (IPTG) concentration (0-2 mM), and controlled batch and fed-batch cultures were tested on a 2-L scale. A concentration of 0.5 mM IPTG was found to be optimal for cell growth and bacteriocin production. Shake flask cultivation of E. coli Origami (DE3) (pLysS/pCR03) gave biomass and bacteriocin yields of 1.54 ± 0.06 g cdw/l and 18 ± 1 mg DvnRV41/l, respectively. Biomass (2.70 ± 0.06 and 6.8 ± 0.6 g cdw/l, respectively) and bacteriocin yields (30 and 74 mg DvnRV41 per liter, respectively) were both increased with batch and fed-batch compared to shake flask cultures. Bacteriocin yields reported in this study are among the highest published for other heterologous expression systems in shake flasks.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Appears in Collections:Publikationen Life Sciences und Facility Management

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