Title: Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures
Authors : Yildirim, Selçuk
Konrad, Daniel
Calvez, Ségolène
Drider, Djamel
Prévost, Hervé
Lacroix, Christophe
Published in : Applied Microbiology and Biotechnology
Volume(Issue) : 77
Issue : 3
Pages : 525
Pages to: 531
Publisher / Ed. Institution : Springer
Issue Date: Dec-2007
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 660.6: Biotechnology
Abstract: To increase the yield of heterologous production of the class II bacteriocin DvnRV41 with Escherichia coli Origami (DE3) (pLysS/pCR03), induction of bacteriocin gene expression was optimized by varying the inducer isopropyl β-d-thiogalactopyranoside (IPTG) concentration (0–2 mM), and controlled batch and fed-batch cultures were tested on a 2-L scale. A concentration of 0.5 mM IPTG was found to be optimal for cell growth and bacteriocin production. Shake flask cultivation of E. coli Origami (DE3) (pLysS/pCR03) gave biomass and bacteriocin yields of 1.54 ± 0.06 g cdw/l and 18 ± 1 mg DvnRV41/l, respectively. Biomass (2.70 ± 0.06 and 6.8 ± 0.6 g cdw/l, respectively) and bacteriocin yields (30 and 74 mg DvnRV41 per liter, respectively) were both increased with batch and fed-batch compared to shake flask cultures. Bacteriocin yields reported in this study are among the highest published for other heterologous expression systems in shake flasks.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Publication type: Article in scientific Journal
DOI : 10.1007/s00253-007-1188-1
ISSN: 0175-7598
URI: https://digitalcollection.zhaw.ch/handle/11475/7075
Appears in Collections:Publikationen Life Sciences und Facility Management

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.