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dc.contributor.authorYildirim, Selçuk-
dc.contributor.authorKonrad, Daniel-
dc.contributor.authorCalvez, Ségolène-
dc.contributor.authorDrider, Djamel-
dc.contributor.authorPrévost, Hervé-
dc.contributor.authorLacroix, Christophe-
dc.date.accessioned2018-06-18T13:55:20Z-
dc.date.available2018-06-18T13:55:20Z-
dc.date.issued2007-12-
dc.identifier.issn0175-7598de_CH
dc.identifier.issn1432-0614de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/7075-
dc.description.abstractTo increase the yield of heterologous production of the class II bacteriocin DvnRV41 with Escherichia coli Origami (DE3) (pLysS/pCR03), induction of bacteriocin gene expression was optimized by varying the inducer isopropyl β-d-thiogalactopyranoside (IPTG) concentration (0-2 mM), and controlled batch and fed-batch cultures were tested on a 2-L scale. A concentration of 0.5 mM IPTG was found to be optimal for cell growth and bacteriocin production. Shake flask cultivation of E. coli Origami (DE3) (pLysS/pCR03) gave biomass and bacteriocin yields of 1.54 ± 0.06 g cdw/l and 18 ± 1 mg DvnRV41/l, respectively. Biomass (2.70 ± 0.06 and 6.8 ± 0.6 g cdw/l, respectively) and bacteriocin yields (30 and 74 mg DvnRV41 per liter, respectively) were both increased with batch and fed-batch compared to shake flask cultures. Bacteriocin yields reported in this study are among the highest published for other heterologous expression systems in shake flasks.de_CH
dc.language.isoende_CH
dc.publisherSpringerde_CH
dc.relation.ispartofApplied Microbiology and Biotechnologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleProduction of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch culturesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Lebensmittel- und Getränkeinnovation (ILGI)de_CH
dc.identifier.doi10.1007/s00253-007-1188-1de_CH
zhaw.funding.euNode_CH
zhaw.issue3de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end531de_CH
zhaw.pages.start525de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume77de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedLM-Verpackungde_CH
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Yildirim, S., Konrad, D., Calvez, S., Drider, D., Prévost, H., & Lacroix, C. (2007). Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures. Applied Microbiology and Biotechnology, 77(3), 525–531. https://doi.org/10.1007/s00253-007-1188-1
Yildirim, S. et al. (2007) ‘Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures’, Applied Microbiology and Biotechnology, 77(3), pp. 525–531. Available at: https://doi.org/10.1007/s00253-007-1188-1.
S. Yildirim, D. Konrad, S. Calvez, D. Drider, H. Prévost, and C. Lacroix, “Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures,” Applied Microbiology and Biotechnology, vol. 77, no. 3, pp. 525–531, Dec. 2007, doi: 10.1007/s00253-007-1188-1.
YILDIRIM, Selçuk, Daniel KONRAD, Ségolène CALVEZ, Djamel DRIDER, Hervé PRÉVOST und Christophe LACROIX, 2007. Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures. Applied Microbiology and Biotechnology. Dezember 2007. Bd. 77, Nr. 3, S. 525–531. DOI 10.1007/s00253-007-1188-1
Yildirim, Selçuk, Daniel Konrad, Ségolène Calvez, Djamel Drider, Hervé Prévost, and Christophe Lacroix. 2007. “Production of Recombinant Bacteriocin Divercin V41 by High Cell Density Escherichia Coli Batch and Fed-Batch Cultures.” Applied Microbiology and Biotechnology 77 (3): 525–31. https://doi.org/10.1007/s00253-007-1188-1.
Yildirim, Selçuk, et al. “Production of Recombinant Bacteriocin Divercin V41 by High Cell Density Escherichia Coli Batch and Fed-Batch Cultures.” Applied Microbiology and Biotechnology, vol. 77, no. 3, Dec. 2007, pp. 525–31, https://doi.org/10.1007/s00253-007-1188-1.


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