| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules |
| Autor/-in: | Pulipparacharuvil, Suprabha Akbar, Mohammed Ray, Sanchali Sevrioukov, Evgueny A. Haberman, Adam S. Rohrer, Jack Krämer, Helmut |
| DOI: | 10.1242/jcs.02502 |
| Erschienen in: | Journal of Cell Science |
| Band(Heft): | 118 |
| Heft: | 16 |
| Seite(n): | 3663 |
| Seiten bis: | 3673 |
| Erscheinungsdatum: | 2005 |
| Verlag / Hrsg. Institution: | The Company of Biologists |
| ISSN: | 0021-9533 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 572: Biochemie |
| Zusammenfassung: | Mutations that disrupt trafficking to lysosomes and lysosome-related organelles cause multiple diseases, including Hermansky-Pudlak syndrome. The Drosophila eye is a model system for analyzing such mutations. The eye-color genes carnation and deep orange encode two subunits of the Vps-C protein complex required for endosomal trafficking and pigment-granule biogenesis. Here we demonstrate that dVps16A (CG8454) encodes another Vps-C subunit. Biochemical experiments revealed a specific interaction between the dVps16A C-terminus and the Sec1/Munc18 homolog Carnation but not its closest homolog, dVps33B. Instead, dVps33B interacted with a related protein, dVps16B (CG18112). Deep orange bound both Vps16 homologs. Like a deep orange null mutation, eye-specific RNAi-induced knockdown of dVps16A inhibited lysosomal delivery of internalized ligands and interfered with biogenesis of pigment granules. Ubiquitous knockdown of dVps16A was lethal. Together, these findings demonstrate that Drosophila Vps16A is essential for lysosomal trafficking. Furthermore, metazoans have two types of Vps-C complexes with non-redundant functions. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/6838 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Pulipparacharuvil, S., Akbar, M., Ray, S., Sevrioukov, E. A., Haberman, A. S., Rohrer, J., & Krämer, H. (2005). Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules. Journal of Cell Science, 118(16), 3663–3673. https://doi.org/10.1242/jcs.02502
Pulipparacharuvil, S. et al. (2005) ‘Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules’, Journal of Cell Science, 118(16), pp. 3663–3673. Available at: https://doi.org/10.1242/jcs.02502.
S. Pulipparacharuvil et al., “Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules,” Journal of Cell Science, vol. 118, no. 16, pp. 3663–3673, 2005, doi: 10.1242/jcs.02502.
PULIPPARACHARUVIL, Suprabha, Mohammed AKBAR, Sanchali RAY, Evgueny A. SEVRIOUKOV, Adam S. HABERMAN, Jack ROHRER und Helmut KRÄMER, 2005. Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules. Journal of Cell Science. 2005. Bd. 118, Nr. 16, S. 3663–3673. DOI 10.1242/jcs.02502
Pulipparacharuvil, Suprabha, Mohammed Akbar, Sanchali Ray, Evgueny A. Sevrioukov, Adam S. Haberman, Jack Rohrer, and Helmut Krämer. 2005. “Drosophila Vps16A Is Required for Trafficking to Lysosomes and Biogenesis of Pigment Granules.” Journal of Cell Science 118 (16): 3663–73. https://doi.org/10.1242/jcs.02502.
Pulipparacharuvil, Suprabha, et al. “Drosophila Vps16A Is Required for Trafficking to Lysosomes and Biogenesis of Pigment Granules.” Journal of Cell Science, vol. 118, no. 16, 2005, pp. 3663–73, https://doi.org/10.1242/jcs.02502.
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