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dc.contributor.authorPulipparacharuvil, Suprabha-
dc.contributor.authorAkbar, Mohammed-
dc.contributor.authorRay, Sanchali-
dc.contributor.authorSevrioukov, Evgueny A.-
dc.contributor.authorHaberman, Adam S.-
dc.contributor.authorRohrer, Jack-
dc.contributor.authorKrämer, Helmut-
dc.date.accessioned2018-06-13T09:46:55Z-
dc.date.available2018-06-13T09:46:55Z-
dc.date.issued2005-
dc.identifier.issn0021-9533de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/6838-
dc.description.abstractMutations that disrupt trafficking to lysosomes and lysosome-related organelles cause multiple diseases, including Hermansky-Pudlak syndrome. The Drosophila eye is a model system for analyzing such mutations. The eye-color genes carnation and deep orange encode two subunits of the Vps-C protein complex required for endosomal trafficking and pigment-granule biogenesis. Here we demonstrate that dVps16A (CG8454) encodes another Vps-C subunit. Biochemical experiments revealed a specific interaction between the dVps16A C-terminus and the Sec1/Munc18 homolog Carnation but not its closest homolog, dVps33B. Instead, dVps33B interacted with a related protein, dVps16B (CG18112). Deep orange bound both Vps16 homologs. Like a deep orange null mutation, eye-specific RNAi-induced knockdown of dVps16A inhibited lysosomal delivery of internalized ligands and interfered with biogenesis of pigment granules. Ubiquitous knockdown of dVps16A was lethal. Together, these findings demonstrate that Drosophila Vps16A is essential for lysosomal trafficking. Furthermore, metazoans have two types of Vps-C complexes with non-redundant functions.de_CH
dc.language.isoende_CH
dc.publisherThe Company of Biologistsde_CH
dc.relation.ispartofJournal of Cell Sciencede_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleDrosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granulesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1242/jcs.02502de_CH
zhaw.funding.euNode_CH
zhaw.issue16de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end3673de_CH
zhaw.pages.start3663de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume118de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedZellphysiologiede_CH
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Pulipparacharuvil, S., Akbar, M., Ray, S., Sevrioukov, E. A., Haberman, A. S., Rohrer, J., & Krämer, H. (2005). Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules. Journal of Cell Science, 118(16), 3663–3673. https://doi.org/10.1242/jcs.02502
Pulipparacharuvil, S. et al. (2005) ‘Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules’, Journal of Cell Science, 118(16), pp. 3663–3673. Available at: https://doi.org/10.1242/jcs.02502.
S. Pulipparacharuvil et al., “Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules,” Journal of Cell Science, vol. 118, no. 16, pp. 3663–3673, 2005, doi: 10.1242/jcs.02502.
PULIPPARACHARUVIL, Suprabha, Mohammed AKBAR, Sanchali RAY, Evgueny A. SEVRIOUKOV, Adam S. HABERMAN, Jack ROHRER und Helmut KRÄMER, 2005. Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules. Journal of Cell Science. 2005. Bd. 118, Nr. 16, S. 3663–3673. DOI 10.1242/jcs.02502
Pulipparacharuvil, Suprabha, Mohammed Akbar, Sanchali Ray, Evgueny A. Sevrioukov, Adam S. Haberman, Jack Rohrer, and Helmut Krämer. 2005. “Drosophila Vps16A Is Required for Trafficking to Lysosomes and Biogenesis of Pigment Granules.” Journal of Cell Science 118 (16): 3663–73. https://doi.org/10.1242/jcs.02502.
Pulipparacharuvil, Suprabha, et al. “Drosophila Vps16A Is Required for Trafficking to Lysosomes and Biogenesis of Pigment Granules.” Journal of Cell Science, vol. 118, no. 16, 2005, pp. 3663–73, https://doi.org/10.1242/jcs.02502.


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