Title: Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules
Authors : Pulipparacharuvil, Suprabha
Akbar, Mohammed
Ray, Sanchali
Sevrioukov, Evgueny A.
Haberman, Adam S.
Rohrer, Jack
Krämer, Helmut
Published in : Journal of Cell Science
Volume(Issue) : 118
Issue : 16
Pages : 3663
Pages to: 3673
Publisher / Ed. Institution : The Company of Biologists
Issue Date: 2005
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 572: Biochemistry
Abstract: Mutations that disrupt trafficking to lysosomes and lysosome-related organelles cause multiple diseases, including Hermansky-Pudlak syndrome. The Drosophila eye is a model system for analyzing such mutations. The eye-color genes carnation and deep orange encode two subunits of the Vps-C protein complex required for endosomal trafficking and pigment-granule biogenesis. Here we demonstrate that dVps16A (CG8454) encodes another Vps-C subunit. Biochemical experiments revealed a specific interaction between the dVps16A C-terminus and the Sec1/Munc18 homolog Carnation but not its closest homolog, dVps33B. Instead, dVps33B interacted with a related protein, dVps16B (CG18112). Deep orange bound both Vps16 homologs. Like a deep orange null mutation, eye-specific RNAi-induced knockdown of dVps16A inhibited lysosomal delivery of internalized ligands and interfered with biogenesis of pigment granules. Ubiquitous knockdown of dVps16A was lethal. Together, these findings demonstrate that Drosophila Vps16A is essential for lysosomal trafficking. Furthermore, metazoans have two types of Vps-C complexes with non-redundant functions.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1242/jcs.02502
ISSN: 0021-9533
URI: https://digitalcollection.zhaw.ch/handle/11475/6838
Appears in Collections:Publikationen Life Sciences und Facility Management

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