Publication type: Article in scientific journal
Type of review: Not specified
Title: Analysis of protein transport to lysosomes
Authors: Schaub, Beat E
Nair, Prashant
Rohrer, Jack
DOI: 10.1002/0471143030.cb1508s27
Published in: Current Protocols in Cell Biology
Volume(Issue): 27
Issue: 1
Issue Date: 1-Jul-2005
Publisher / Ed. Institution: Wiley
ISSN: 1934-2500
Language: English
Subject (DDC): 571: Physiology and related subjects
Abstract: Lysosomes are terminal degradative organelles that are found in all higher eukaryotic cells. The biogenesis of lysosomes involves the transport of various acid hydrolases and transmembrane glycoproteins from their site of synthesis in the endoplasmic reticulum through the biosynthetic and endocytic pathways. Protein transport to lysosomes can be studied by a combination of techniques based on the separation of intracellular organelles. Percoll density gradient centrifugation has long been the method of choice for separating lysosomes from other organelles in cell homogenates, and accordingly, this unit describes protocols for obtaining reasonably pure lysosomal fractions from mammalian cells using Percoll density gradient separation.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in Collections:Publikationen Life Sciences und Facility Management

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