| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Keine Angabe |
| Titel: | Analysis of protein transport to lysosomes |
| Autor/-in: | Schaub, Beat E Nair, Prashant Rohrer, Jack |
| DOI: | 10.1002/0471143030.cb1508s27 |
| Erschienen in: | Current Protocols in Cell Biology |
| Band(Heft): | 27 |
| Heft: | 1 |
| Erscheinungsdatum: | 1-Jul-2005 |
| Verlag / Hrsg. Institution: | Wiley |
| ISSN: | 1934-2500 1934-2616 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 571: Physiologie und verwandte Themen |
| Zusammenfassung: | Lysosomes are terminal degradative organelles that are found in all higher eukaryotic cells. The biogenesis of lysosomes involves the transport of various acid hydrolases and transmembrane glycoproteins from their site of synthesis in the endoplasmic reticulum through the biosynthetic and endocytic pathways. Protein transport to lysosomes can be studied by a combination of techniques based on the separation of intracellular organelles. Percoll density gradient centrifugation has long been the method of choice for separating lysosomes from other organelles in cell homogenates, and accordingly, this unit describes protocols for obtaining reasonably pure lysosomal fractions from mammalian cells using Percoll density gradient separation. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/6800 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Schaub, B. E., Nair, P., & Rohrer, J. (2005). Analysis of protein transport to lysosomes. Current Protocols in Cell Biology, 27(1). https://doi.org/10.1002/0471143030.cb1508s27
Schaub, B.E., Nair, P. and Rohrer, J. (2005) ‘Analysis of protein transport to lysosomes’, Current Protocols in Cell Biology, 27(1). Available at: https://doi.org/10.1002/0471143030.cb1508s27.
B. E. Schaub, P. Nair, and J. Rohrer, “Analysis of protein transport to lysosomes,” Current Protocols in Cell Biology, vol. 27, no. 1, Jul. 2005, doi: 10.1002/0471143030.cb1508s27.
SCHAUB, Beat E, Prashant NAIR und Jack ROHRER, 2005. Analysis of protein transport to lysosomes. Current Protocols in Cell Biology. 1 Juli 2005. Bd. 27, Nr. 1. DOI 10.1002/0471143030.cb1508s27
Schaub, Beat E, Prashant Nair, and Jack Rohrer. 2005. “Analysis of Protein Transport to Lysosomes.” Current Protocols in Cell Biology 27 (1). https://doi.org/10.1002/0471143030.cb1508s27.
Schaub, Beat E., et al. “Analysis of Protein Transport to Lysosomes.” Current Protocols in Cell Biology, vol. 27, no. 1, July 2005, https://doi.org/10.1002/0471143030.cb1508s27.
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