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https://doi.org/10.21256/zhaw-1748| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Open peer review |
| Titel: | Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration |
| Autor/-in: | Mauth, C Bono, Epifania Haas, S Paesold, G. Wiese, H. Maier, G. Boos, N. Graf-Hausner, Ursula |
| DOI: | 10.21256/zhaw-1748 10.22203/eCM.v018a03 |
| Erschienen in: | European Cells & Materials |
| Band(Heft): | 18 |
| Seite(n): | 27 |
| Seiten bis: | 39 |
| Erscheinungsdatum: | 2009 |
| Verlag / Hrsg. Institution: | Swiss Society for Biomaterials |
| ISSN: | 1473-2262 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 610: Medizin und Gesundheit |
| Zusammenfassung: | Nowadays, intervertebral disc (IVD) degeneration is one of the principal causes of low back pain involving high expense within the health care system. The long-term goal is the development of a medical treatment modality focused on a more biological regeneration of the inner nucleus pulposus (NP). Hence, interest in the endoscopic implantation of an injectable material took center stage in the recent past. We report on the development of a novel polyurethane (PU) scaffold as a mechanically stable carrier system for the reimplantation of expanded autologous IVD-derived cells (disc cells) to stimulate regenerative processes and restore the chondrocyte-like tissue within the NP. Primary human disc cells were seeded into newly developed PU spheroids which were subsequently encapsulated in fibrin hydrogel. The study aims to analyze adhesion properties, proliferation capacity and phenotypic characterization of these cells. Polymerase chain reaction was carried out to detect the expression of genes specifically expressed by native IVD cells. Biochemical analyses showed an increased DNA content, and a progressive enhancement of total collagen and glycosaminoglycans (GAG) was observed during cell culture. The results suggest the synthesis of an appropriate extracellular matrix as well as a stable mRNA expression of chondrogenic and/or NP specific markers. In conclusion, the data presented indicate an alternative medical approach to current treatment options of degenerated IVD tissue. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/3270 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | School of Engineering |
| Enthalten in den Sammlungen: | Publikationen School of Engineering |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2009_Mauth_Cell-Seeded_Polyurethane_EuropeanCells.pdf | 552.17 kB | Adobe PDF |  Öffnen/Anzeigen |
Zur Langanzeige
Mauth, C., Bono, E., Haas, S., Paesold, G., Wiese, H., Maier, G., Boos, N., & Graf-Hausner, U. (2009). Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration. European Cells & Materials, 18, 27–39. https://doi.org/10.21256/zhaw-1748
Mauth, C. et al. (2009) ‘Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration’, European Cells & Materials, 18, pp. 27–39. Available at: https://doi.org/10.21256/zhaw-1748.
C. Mauth et al., “Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration,” European Cells & Materials, vol. 18, pp. 27–39, 2009, doi: 10.21256/zhaw-1748.
MAUTH, C, Epifania BONO, S HAAS, G. PAESOLD, H. WIESE, G. MAIER, N. BOOS und Ursula GRAF-HAUSNER, 2009. Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration. European Cells & Materials. 2009. Bd. 18, S. 27–39. DOI 10.21256/zhaw-1748
Mauth, C, Epifania Bono, S Haas, G. Paesold, H. Wiese, G. Maier, N. Boos, and Ursula Graf-Hausner. 2009. “Cell-Seeded Polyurethane-Fibrin Structures : A Possible System for Intervertebral Disc Regeneration.” European Cells & Materials 18: 27–39. https://doi.org/10.21256/zhaw-1748.
Mauth, C., et al. “Cell-Seeded Polyurethane-Fibrin Structures : A Possible System for Intervertebral Disc Regeneration.” European Cells & Materials, vol. 18, 2009, pp. 27–39, https://doi.org/10.21256/zhaw-1748.
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