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Title: Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration
Authors : Mauth, C
Bono, Epifania
Haas, S
Paesold, G.
Wiese, H.
Maier, G.
Boos, N.
Graf-Hausner, Ursula
Published in : European Cells and Materials
Volume(Issue) : 18
Pages : 27
Pages to: 39
Publisher / Ed. Institution : Swiss Society for Biomaterials
Issue Date: 2009
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 610: Medicine and health
Abstract: Nowadays, intervertebral disc (IVD) degeneration is one of the principal causes of low back pain involving high expense within the health care system. The long-term goal is the development of a medical treatment modality focused on a more biological regeneration of the inner nucleus pulposus (NP). Hence, interest in the endoscopic implantation of an injectable material took center stage in the recent past. We report on the development of a novel polyurethane (PU) scaffold as a mechanically stable carrier system for the reimplantation of expanded autologous IVD-derived cells (disc cells) to stimulate regenerative processes and restore the chondrocyte-like tissue within the NP. Primary human disc cells were seeded into newly developed PU spheroids which were subsequently encapsulated in fibrin hydrogel. The study aims to analyze adhesion properties, proliferation capacity and phenotypic characterization of these cells. Polymerase chain reaction was carried out to detect the expression of genes specifically expressed by native IVD cells. Biochemical analyses showed an increased DNA content, and a progressive enhancement of total collagen and glycosaminoglycans (GAG) was observed during cell culture. The results suggest the synthesis of an appropriate extracellular matrix as well as a stable mRNA expression of chondrogenic and/or NP specific markers. In conclusion, the data presented indicate an alternative medical approach to current treatment options of degenerated IVD tissue.
Departement: School of Engineering
Publication type: Article in scientific Journal
DOI : 10.22203/eCM.v018a03
ISSN: 1473-2262
Appears in Collections:Publikationen School of Engineering

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