Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration

Mauth, C; Bono, Epifania; Haas, S; Paesold, G.; Wiese, H.; Maier, G.; Boos, N.; Graf-Hausner, Ursula

doi:10.21256/zhaw-1748

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1748

Publication type:	Article in scientific journal
Type of review:	Open peer review
Title:	Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration
Authors:	Mauth, C Bono, Epifania Haas, S Paesold, G. Wiese, H. Maier, G. Boos, N. Graf-Hausner, Ursula
DOI:	10.21256/zhaw-1748 10.22203/eCM.v018a03
Published in:	European Cells & Materials
Volume(Issue):	18
Page(s):	27
Pages to:	39
Issue Date:	2009
Publisher / Ed. Institution:	Swiss Society for Biomaterials
ISSN:	1473-2262
Language:	English
Subject (DDC):	610: Medicine and health
Abstract:	Nowadays, intervertebral disc (IVD) degeneration is one of the principal causes of low back pain involving high expense within the health care system. The long-term goal is the development of a medical treatment modality focused on a more biological regeneration of the inner nucleus pulposus (NP). Hence, interest in the endoscopic implantation of an injectable material took center stage in the recent past. We report on the development of a novel polyurethane (PU) scaffold as a mechanically stable carrier system for the reimplantation of expanded autologous IVD-derived cells (disc cells) to stimulate regenerative processes and restore the chondrocyte-like tissue within the NP. Primary human disc cells were seeded into newly developed PU spheroids which were subsequently encapsulated in fibrin hydrogel. The study aims to analyze adhesion properties, proliferation capacity and phenotypic characterization of these cells. Polymerase chain reaction was carried out to detect the expression of genes specifically expressed by native IVD cells. Biochemical analyses showed an increased DNA content, and a progressive enhancement of total collagen and glycosaminoglycans (GAG) was observed during cell culture. The results suggest the synthesis of an appropriate extracellular matrix as well as a stable mRNA expression of chondrogenic and/or NP specific markers. In conclusion, the data presented indicate an alternative medical approach to current treatment options of degenerated IVD tissue.
URI:	https://digitalcollection.zhaw.ch/handle/11475/3270
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	School of Engineering
Appears in collections:	Publikationen School of Engineering

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Mauth, C., Bono, E., Haas, S., Paesold, G., Wiese, H., Maier, G., Boos, N., & Graf-Hausner, U. (2009). Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration. European Cells & Materials, 18, 27–39. https://doi.org/10.21256/zhaw-1748

Mauth, C. et al. (2009) ‘Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration’, European Cells & Materials, 18, pp. 27–39. Available at: https://doi.org/10.21256/zhaw-1748.

C. Mauth et al., “Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration,” European Cells & Materials, vol. 18, pp. 27–39, 2009, doi: 10.21256/zhaw-1748.

MAUTH, C, Epifania BONO, S HAAS, G. PAESOLD, H. WIESE, G. MAIER, N. BOOS und Ursula GRAF-HAUSNER, 2009. Cell-seeded polyurethane-fibrin structures : a possible system for intervertebral disc regeneration. European Cells & Materials. 2009. Bd. 18, S. 27–39. DOI 10.21256/zhaw-1748

Mauth, C, Epifania Bono, S Haas, G. Paesold, H. Wiese, G. Maier, N. Boos, and Ursula Graf-Hausner. 2009. “Cell-Seeded Polyurethane-Fibrin Structures : A Possible System for Intervertebral Disc Regeneration.” European Cells & Materials 18: 27–39. https://doi.org/10.21256/zhaw-1748.

Mauth, C., et al. “Cell-Seeded Polyurethane-Fibrin Structures : A Possible System for Intervertebral Disc Regeneration.” European Cells & Materials, vol. 18, 2009, pp. 27–39, https://doi.org/10.21256/zhaw-1748.