| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale |
| Autor/-in: | Blaschczok, Katharina Löffelholz, Christian Eibl-Schindler, Regine Eibl, Dieter |
| DOI: | 10.1002/elsc.201300069 |
| Erschienen in: | Engineering in Life Sciences |
| Band(Heft): | 14 |
| Heft: | 3 |
| Seite(n): | 327 |
| Seiten bis: | 331 |
| Erscheinungsdatum: | Mai-2014 |
| Verlag / Hrsg. Institution: | Wiley |
| ISSN: | 1618-2863 |
| Sprache: | Englisch |
| Schlagwörter: | Biphasic protein expression; Chinese hamster ovary cells; Hollow fiber module; SEAP (secreted alkaline phosphatase); Stirred single-use bioreactor |
| Fachgebiet (DDC): | 660: Technische Chemie |
| Zusammenfassung: | The increasing implementation of single-use bioreactors arrived hand-in-hand with the development of new technologies contributing to increased productivity, process flexibility, and additional savings in time and costs. As a result, hollow fiber technology has recently gained renewed interest in upstream processing. Using a Chinese hamster ovary cell line in a biphasic protein production process with chemically defined minimal culture media, we combined Sartorius Stedim's BIOSTAT STR 50 L with GE Healthcare Life Sciences’ reusable Hollow Fiber Cartridge CFP-6-D-55A. After a 3-day feeding growth phase, secretion of the model protein secreted alkaline phosphatase (SEAP) was introduced by replacing the growth medium with production medium using cross-flow filtration. The process was then continued and harvested as a batch with temperature shift. High cell densities exceeding 1 × 107 cells mL−1 were achieved 5 days post inoculation and maximum secreted alkaline phosphatase activities of 24 U mL−1 11 days post inoculation. Our results showed that a further decrease in processing time is possible by reducing the number of diafiltration steps from three to two. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/2929 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Blaschczok, K., Löffelholz, C., Eibl-Schindler, R., & Eibl, D. (2014). Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale. Engineering in Life Sciences, 14(3), 327–331. https://doi.org/10.1002/elsc.201300069
Blaschczok, K. et al. (2014) ‘Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale’, Engineering in Life Sciences, 14(3), pp. 327–331. Available at: https://doi.org/10.1002/elsc.201300069.
K. Blaschczok, C. Löffelholz, R. Eibl-Schindler, and D. Eibl, “Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale,” Engineering in Life Sciences, vol. 14, no. 3, pp. 327–331, May 2014, doi: 10.1002/elsc.201300069.
BLASCHCZOK, Katharina, Christian LÖFFELHOLZ, Regine EIBL-SCHINDLER und Dieter EIBL, 2014. Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale. Engineering in Life Sciences. Mai 2014. Bd. 14, Nr. 3, S. 327–331. DOI 10.1002/elsc.201300069
Blaschczok, Katharina, Christian Löffelholz, Regine Eibl-Schindler, and Dieter Eibl. 2014. “Combining Single-Use Stirred Bioreactor with Standard Cross-Flow Technology in Biphasic Protein Production Processes at Pilot Scale.” Engineering in Life Sciences 14 (3): 327–31. https://doi.org/10.1002/elsc.201300069.
Blaschczok, Katharina, et al. “Combining Single-Use Stirred Bioreactor with Standard Cross-Flow Technology in Biphasic Protein Production Processes at Pilot Scale.” Engineering in Life Sciences, vol. 14, no. 3, May 2014, pp. 327–31, https://doi.org/10.1002/elsc.201300069.
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