Title: Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale
Authors : Blaschczok, Katharina
Löffelholz, Christian
Eibl-Schindler, Regine
Eibl, Dieter
Published in : Engineering in Life Sciences
Volume(Issue) : 14
Issue : 3
Pages : 327
Pages to: 331
Publisher / Ed. Institution : Wiley
Issue Date: May-2014
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subjects : Biphasic protein expression; Chinese hamster ovary cells; Hollow fiber module; SEAP (secreted alkaline phosphatase); Stirred single-use bioreactor
Subject (DDC) : 660: Chemical engineering
Abstract: The increasing implementation of single-use bioreactors arrived hand-in-hand with the development of new technologies contributing to increased productivity, process flexibility, and additional savings in time and costs. As a result, hollow fiber technology has recently gained renewed interest in upstream processing. Using a Chinese hamster ovary cell line in a biphasic protein production process with chemically defined minimal culture media, we combined Sartorius Stedim's BIOSTAT STR 50 L with GE Healthcare Life Sciences’ reusable Hollow Fiber Cartridge CFP-6-D-55A. After a 3-day feeding growth phase, secretion of the model protein secreted alkaline phosphatase (SEAP) was introduced by replacing the growth medium with production medium using cross-flow filtration. The process was then continued and harvested as a batch with temperature shift. High cell densities exceeding 1 × 107 cells mL−1 were achieved 5 days post inoculation and maximum secreted alkaline phosphatase activities of 24 U mL−1 11 days post inoculation. Our results showed that a further decrease in processing time is possible by reducing the number of diafiltration steps from three to two.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific journal
DOI : 10.1002/elsc.201300069
ISSN: 1618-2863
URI: https://digitalcollection.zhaw.ch/handle/11475/2929
Appears in Collections:Publikationen Life Sciences und Facility Management

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