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  4. Publikationen Life Sciences und Facility Management
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dc.contributor.authorBlaschczok, Katharina-
dc.contributor.authorLöffelholz, Christian-
dc.contributor.authorEibl-Schindler, Regine-
dc.contributor.authorEibl, Dieter-
dc.date.accessioned2018-02-22T14:37:12Z-
dc.date.available2018-02-22T14:37:12Z-
dc.date.issued2014-05-
dc.identifier.issn1618-2863de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/2929-
dc.description.abstractThe increasing implementation of single-use bioreactors arrived hand-in-hand with the development of new technologies contributing to increased productivity, process flexibility, and additional savings in time and costs. As a result, hollow fiber technology has recently gained renewed interest in upstream processing. Using a Chinese hamster ovary cell line in a biphasic protein production process with chemically defined minimal culture media, we combined Sartorius Stedim's BIOSTAT STR 50 L with GE Healthcare Life Sciences’ reusable Hollow Fiber Cartridge CFP-6-D-55A. After a 3-day feeding growth phase, secretion of the model protein secreted alkaline phosphatase (SEAP) was introduced by replacing the growth medium with production medium using cross-flow filtration. The process was then continued and harvested as a batch with temperature shift. High cell densities exceeding 1 × 107 cells mL−1 were achieved 5 days post inoculation and maximum secreted alkaline phosphatase activities of 24 U mL−1 11 days post inoculation. Our results showed that a further decrease in processing time is possible by reducing the number of diafiltration steps from three to two.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofEngineering in Life Sciencesde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectBiphasic protein expressionde_CH
dc.subjectChinese hamster ovary cellsde_CH
dc.subjectHollow fiber modulede_CH
dc.subjectSEAP (secreted alkaline phosphatase)de_CH
dc.subjectStirred single-use bioreactorde_CH
dc.subject.ddc660: Technische Chemiede_CH
dc.titleCombining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scalede_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1002/elsc.201300069de_CH
zhaw.funding.euNode_CH
zhaw.issue3de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end331de_CH
zhaw.pages.start327de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume14de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Blaschczok, K., Löffelholz, C., Eibl-Schindler, R., & Eibl, D. (2014). Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale. Engineering in Life Sciences, 14(3), 327–331. https://doi.org/10.1002/elsc.201300069
Blaschczok, K. et al. (2014) ‘Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale’, Engineering in Life Sciences, 14(3), pp. 327–331. Available at: https://doi.org/10.1002/elsc.201300069.
K. Blaschczok, C. Löffelholz, R. Eibl-Schindler, and D. Eibl, “Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale,” Engineering in Life Sciences, vol. 14, no. 3, pp. 327–331, May 2014, doi: 10.1002/elsc.201300069.
BLASCHCZOK, Katharina, Christian LÖFFELHOLZ, Regine EIBL-SCHINDLER und Dieter EIBL, 2014. Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale. Engineering in Life Sciences. Mai 2014. Bd. 14, Nr. 3, S. 327–331. DOI 10.1002/elsc.201300069
Blaschczok, Katharina, Christian Löffelholz, Regine Eibl-Schindler, and Dieter Eibl. 2014. “Combining Single-Use Stirred Bioreactor with Standard Cross-Flow Technology in Biphasic Protein Production Processes at Pilot Scale.” Engineering in Life Sciences 14 (3): 327–31. https://doi.org/10.1002/elsc.201300069.
Blaschczok, Katharina, et al. “Combining Single-Use Stirred Bioreactor with Standard Cross-Flow Technology in Biphasic Protein Production Processes at Pilot Scale.” Engineering in Life Sciences, vol. 14, no. 3, May 2014, pp. 327–31, https://doi.org/10.1002/elsc.201300069.


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