Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Fluorescence reporters for phosphodiesterase activity
Authors : Berkessel, Albrecht
Riedl, Rainer
DOI : 10.1002/anie.199714811
Published in : Angewandte Chemie: International Edition
Volume(Issue) : 36
Issue : 13-14
Pages : 1481
Pages to: 1483
Issue Date: 1997
Publisher / Ed. Institution : Wiley
ISSN: 1433-7851
Language : English
Subjects : Fluorescence Reporter
Subject (DDC) : 615: Pharmacology and therapeutics
Abstract: A sensitive indicator of nuclease activity is the fluorescence behavior of phosphodiesters 1 and 2. Although 1 and 2 do not fluoresce due to intramolecular quenching, the strongly fluorescent naphthyl phosphates and naphthols are liberated upon hydrolysis. This gives rise to an extremely strong increase in fluorescence intensity. Combined application of 1 and 2 allows detection of phosphatase in addition to phosphodiesterase activity. f = fluorophore, q = quencher.
Fulltext version : Published version
License (according to publishing contract) : Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in Collections:Publikationen Life Sciences und Facility Management

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