|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Validation of N-Light™ salmonella risk test kit for detection of Salmonella spp. on environmental surfaces : AOAC Performance Tested MethodsSM 072204|
De Oliveira, Déborah
|Published in:||Journal of AOAC INTERNATIONAL|
|Publisher / Ed. Institution:||Oxford University Press|
|Subjects:||Salmonella; Environmental monitoring|
|Subject (DDC):||363: Environmental and security problems|
|Abstract:||Background: The NEMIS N-Light™ Salmonella Risk method utilizes chemiluminescence designed for the qualitative detection of Salmonella spp. from environmental surface samples. Objective: To validate the N-Light™ Salmonella Risk assay using independent and method developer validation studies according the AOAC Performance Tested MethodsSM (PTM) Program for the detection of Salmonella spp. On stainless steel, polystyrene, and ceramic environmental surfaces. Methods: The N-Light Salmonella Risk assay was evaluated in a matrix study in comparison to the ISO 6579-1:2017 method (“Microbiology of the food chain – Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 1: Detection of Salmonella spp.”) using an unpaired study design. Additional PTM studies performed were inclusivity/exclusivity, robustness, product consistency and stability. Results: The N-Light Salmonella Risk assay demonstrated a specific detection of all Salmonella strains tested. In the matrix study the N-Light Salmonella Risk assay showed no significant differences between presumptive and confirmed results, or between candidate and reference method results on the three surfaces evaluated. Data for additional PTM studies met acceptance criteria requirements. Conclusions: The NEMIS Technologies N-Light Salmonella Risk assay is an effective method for the qualitative detection of Salmonella from stainless steel, polystyrene, and ceramic environmental surfaces. Highlights: The NEMIS Technologies N-Light Salmonella Risk assay which is the first chemiluminescence based detection system applying on a novel, patented dioxetane compound allows an easy and rapid detection of Salmonella.|
|Fulltext version:||Published version|
|License (according to publishing contract):||CC BY-NC-ND 4.0: Attribution - Non commercial - No derivatives 4.0 International|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Food and Beverage Innovation (ILGI)|
|Appears in collections:||Publikationen Life Sciences und Facility Management|
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Desroche, N., De Oliveira, D., Imhaus, A.-F., Hupfeld, M., & Fieseler, L. (2023). Validation of N-Light™ salmonella risk test kit for detection of Salmonella spp. on environmental surfaces : AOAC Performance Tested MethodsSM 072204. Journal of AOAC INTERNATIONAL. https://doi.org/10.1093/jaoacint/qsad017
Desroche, N. et al. (2023) ‘Validation of N-Light™ salmonella risk test kit for detection of Salmonella spp. on environmental surfaces : AOAC Performance Tested MethodsSM 072204’, Journal of AOAC INTERNATIONAL [Preprint]. Available at: https://doi.org/10.1093/jaoacint/qsad017.
N. Desroche, D. De Oliveira, A.-F. Imhaus, M. Hupfeld, and L. Fieseler, “Validation of N-Light™ salmonella risk test kit for detection of Salmonella spp. on environmental surfaces : AOAC Performance Tested MethodsSM 072204,” Journal of AOAC INTERNATIONAL, Jan. 2023, doi: 10.1093/jaoacint/qsad017.
Desroche, Nicolas, et al. “Validation of N-Light™ Salmonella Risk Test Kit for Detection of Salmonella Spp. On Environmental Surfaces : AOAC Performance Tested MethodsSM 072204.” Journal of AOAC INTERNATIONAL, Jan. 2023, https://doi.org/10.1093/jaoacint/qsad017.
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