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https://doi.org/10.21256/zhaw-27147| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Validation of N-Light™ salmonella risk test kit for detection of Salmonella spp. on environmental surfaces : AOAC Performance Tested MethodsSM 072204 |
| Autor/-in: | Desroche, Nicolas De Oliveira, Déborah Imhaus, Anne-Flore Hupfeld, Mario Fieseler, Lars |
| et. al: | No |
| DOI: | 10.1093/jaoacint/qsad017 10.21256/zhaw-27147 |
| Erschienen in: | Journal of AOAC INTERNATIONAL |
| Band(Heft): | 106 |
| Heft: | 4 |
| Seite(n): | 939 |
| Seiten bis: | 948 |
| Erscheinungsdatum: | 20-Jan-2023 |
| Verlag / Hrsg. Institution: | Oxford University Press |
| ISSN: | 1060-3271 1944-7922 |
| Sprache: | Englisch |
| Schlagwörter: | Salmonella; Environmental monitoring |
| Fachgebiet (DDC): | 363: Umwelt- und Sicherheitsprobleme |
| Zusammenfassung: | Background: The NEMIS N-Light™ Salmonella Risk method utilizes chemiluminescence designed for the qualitative detection of Salmonella spp. from environmental surface samples. Objective: To validate the N-Light™ Salmonella Risk assay using independent and method developer validation studies according the AOAC Performance Tested MethodsSM (PTM) Program for the detection of Salmonella spp. On stainless steel, polystyrene, and ceramic environmental surfaces. Methods: The N-Light Salmonella Risk assay was evaluated in a matrix study in comparison to the ISO 6579-1:2017 method (“Microbiology of the food chain – Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 1: Detection of Salmonella spp.”) using an unpaired study design. Additional PTM studies performed were inclusivity/exclusivity, robustness, product consistency and stability. Results: The N-Light Salmonella Risk assay demonstrated a specific detection of all Salmonella strains tested. In the matrix study the N-Light Salmonella Risk assay showed no significant differences between presumptive and confirmed results, or between candidate and reference method results on the three surfaces evaluated. Data for additional PTM studies met acceptance criteria requirements. Conclusions: The NEMIS Technologies N-Light Salmonella Risk assay is an effective method for the qualitative detection of Salmonella from stainless steel, polystyrene, and ceramic environmental surfaces. Highlights: The NEMIS Technologies N-Light Salmonella Risk assay which is the first chemiluminescence based detection system applying on a novel, patented dioxetane compound allows an easy and rapid detection of Salmonella. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/27147 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY-NC-ND 4.0: Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Lebensmittel- und Getränkeinnovation (ILGI) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2023_Desroche-etal_Validation-of-N-Light-Salmonella-risk-test-kit.pdf | 330.57 kB | Adobe PDF |  Öffnen/Anzeigen |
Zur Langanzeige
Desroche, N., De Oliveira, D., Imhaus, A.-F., Hupfeld, M., & Fieseler, L. (2023). Validation of N-Light™ salmonella risk test kit for detection of Salmonella spp. on environmental surfaces : AOAC Performance Tested MethodsSM 072204. Journal of AOAC INTERNATIONAL, 106(4), 939–948. https://doi.org/10.1093/jaoacint/qsad017
Desroche, N. et al. (2023) ‘Validation of N-Light™ salmonella risk test kit for detection of Salmonella spp. on environmental surfaces : AOAC Performance Tested MethodsSM 072204’, Journal of AOAC INTERNATIONAL, 106(4), pp. 939–948. Available at: https://doi.org/10.1093/jaoacint/qsad017.
N. Desroche, D. De Oliveira, A.-F. Imhaus, M. Hupfeld, and L. Fieseler, “Validation of N-Light™ salmonella risk test kit for detection of Salmonella spp. on environmental surfaces : AOAC Performance Tested MethodsSM 072204,” Journal of AOAC INTERNATIONAL, vol. 106, no. 4, pp. 939–948, Jan. 2023, doi: 10.1093/jaoacint/qsad017.
DESROCHE, Nicolas, Déborah DE OLIVEIRA, Anne-Flore IMHAUS, Mario HUPFELD und Lars FIESELER, 2023. Validation of N-Light™ salmonella risk test kit for detection of Salmonella spp. on environmental surfaces : AOAC Performance Tested MethodsSM 072204. Journal of AOAC INTERNATIONAL. 20 Januar 2023. Bd. 106, Nr. 4, S. 939–948. DOI 10.1093/jaoacint/qsad017
Desroche, Nicolas, Déborah De Oliveira, Anne-Flore Imhaus, Mario Hupfeld, and Lars Fieseler. 2023. “Validation of N-Light™ Salmonella Risk Test Kit for Detection of Salmonella Spp. On Environmental Surfaces : AOAC Performance Tested MethodsSM 072204.” Journal of AOAC INTERNATIONAL 106 (4): 939–48. https://doi.org/10.1093/jaoacint/qsad017.
Desroche, Nicolas, et al. “Validation of N-Light™ Salmonella Risk Test Kit for Detection of Salmonella Spp. On Environmental Surfaces : AOAC Performance Tested MethodsSM 072204.” Journal of AOAC INTERNATIONAL, vol. 106, no. 4, Jan. 2023, pp. 939–48, https://doi.org/10.1093/jaoacint/qsad017.
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