Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding

Vo, Andy N.; Kundu, Srikanya; Strong, Caroline; Jung, Olive; Lee, Emily; Song, Min Jae; Boutin, Molly E.; Raghunath, Michael; Ferrer, Marc

doi:10.3390/cells11142131

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26839

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding
Authors:	Vo, Andy N. Kundu, Srikanya Strong, Caroline Jung, Olive Lee, Emily Song, Min Jae Boutin, Molly E. Raghunath, Michael Ferrer, Marc
et. al:	No
DOI:	10.3390/cells11142131 10.21256/zhaw-26839
Published in:	Cells
Volume(Issue):	11
Issue:	14
Page(s):	2131
Issue Date:	2022
Publisher / Ed. Institution:	MDPI
ISSN:	2073-4409
Language:	English
Subjects:	Drug testing; Extracellular matrix; Human iPSC derived astrocyte and dopaminergic neurons; Macromolecular crowding; Cell differentiation; Coculture technique; Humans; Macromolecular substance; Cell culture technique; Extracellular matrix
Subject (DDC):	610.28: Biomedicine, biomedical engineering
Abstract:	The neuroglial extracellular matrix (ECM) provides critical support and physiological cues for the proper growth, differentiation, and function of neuronal cells in the brain. However, in most in vitro settings that study neural physiology, cells are grown as monolayers on stiff surfaces that maximize adhesion and proliferation, and, therefore, they lack the physiological cues that ECM in native neuronal tissues provides. Macromolecular crowding (MMC) is a biophysical phenomenon based on the principle of excluded volume that can be harnessed to induce native ECM deposition by cells in culture. Here, we show that MMC using two species of Ficoll with vitamin C supplementation significantly boosts deposition of relevant brain ECM by cultured human astrocytes. Dopaminergic neurons cocultured on this astrocyte-ECM bed prepared under MMC treatment showed longer and denser neuronal extensions, a higher number of pre ad post synaptic contacts, and increased physiological activity, as evidenced by higher frequency calcium oscillation, compared to standard coculture conditions. When the pharmacological activity of various compounds was tested on MMC-treated cocultures, their responses were enhanced, and for apomorphine, a D2-receptor agonist, it was inverted in comparison to control cell culture conditions, thus emulating responses observed in in vivo settings. These results indicate that macromolecular crowding can harness the ECM-building potential of human astrocytes in vitro forming an ultra-flat 3D microenvironment that makes neural cultures more physiological and pharmacological relevant.
Further description:	Swissnex Austausch
URI:	https://digitalcollection.zhaw.ch/handle/11475/26839
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

Files in This Item:

File	Description	Size	Format
2022_Vo-etal_Enhancement-neuroglial_extracellular-matrix-formation-macromolecular-crowding _MDPI.pdf		6.88 MB	Adobe PDF	View/Open

Show full item record

Vo, A. N., Kundu, S., Strong, C., Jung, O., Lee, E., Song, M. J., Boutin, M. E., Raghunath, M., & Ferrer, M. (2022). Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding. Cells, 11(14), 2131. https://doi.org/10.3390/cells11142131

Vo, A.N. et al. (2022) ‘Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding’, Cells, 11(14), p. 2131. Available at: https://doi.org/10.3390/cells11142131.

A. N. Vo et al., “Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding,” Cells, vol. 11, no. 14, p. 2131, 2022, doi: 10.3390/cells11142131.

VO, Andy N., Srikanya KUNDU, Caroline STRONG, Olive JUNG, Emily LEE, Min Jae SONG, Molly E. BOUTIN, Michael RAGHUNATH und Marc FERRER, 2022. Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding. Cells. 2022. Bd. 11, Nr. 14, S. 2131. DOI 10.3390/cells11142131

Vo, Andy N., Srikanya Kundu, Caroline Strong, Olive Jung, Emily Lee, Min Jae Song, Molly E. Boutin, Michael Raghunath, and Marc Ferrer. 2022. “Enhancement of Neuroglial Extracellular Matrix Formation and Physiological Activity of Dopaminergic Neural Cocultures by Macromolecular Crowding.” Cells 11 (14): 2131. https://doi.org/10.3390/cells11142131.

Vo, Andy N., et al. “Enhancement of Neuroglial Extracellular Matrix Formation and Physiological Activity of Dopaminergic Neural Cocultures by Macromolecular Crowding.” Cells, vol. 11, no. 14, 2022, p. 2131, https://doi.org/10.3390/cells11142131.