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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26839
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dc.contributor.authorVo, Andy N.-
dc.contributor.authorKundu, Srikanya-
dc.contributor.authorStrong, Caroline-
dc.contributor.authorJung, Olive-
dc.contributor.authorLee, Emily-
dc.contributor.authorSong, Min Jae-
dc.contributor.authorBoutin, Molly E.-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorFerrer, Marc-
dc.date.accessioned2023-02-09T11:36:58Z-
dc.date.available2023-02-09T11:36:58Z-
dc.date.issued2022-
dc.identifier.issn2073-4409de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/26839-
dc.descriptionSwissnex Austauschde_CH
dc.description.abstractThe neuroglial extracellular matrix (ECM) provides critical support and physiological cues for the proper growth, differentiation, and function of neuronal cells in the brain. However, in most in vitro settings that study neural physiology, cells are grown as monolayers on stiff surfaces that maximize adhesion and proliferation, and, therefore, they lack the physiological cues that ECM in native neuronal tissues provides. Macromolecular crowding (MMC) is a biophysical phenomenon based on the principle of excluded volume that can be harnessed to induce native ECM deposition by cells in culture. Here, we show that MMC using two species of Ficoll with vitamin C supplementation significantly boosts deposition of relevant brain ECM by cultured human astrocytes. Dopaminergic neurons cocultured on this astrocyte-ECM bed prepared under MMC treatment showed longer and denser neuronal extensions, a higher number of pre ad post synaptic contacts, and increased physiological activity, as evidenced by higher frequency calcium oscillation, compared to standard coculture conditions. When the pharmacological activity of various compounds was tested on MMC-treated cocultures, their responses were enhanced, and for apomorphine, a D2-receptor agonist, it was inverted in comparison to control cell culture conditions, thus emulating responses observed in in vivo settings. These results indicate that macromolecular crowding can harness the ECM-building potential of human astrocytes in vitro forming an ultra-flat 3D microenvironment that makes neural cultures more physiological and pharmacological relevant.de_CH
dc.language.isoende_CH
dc.publisherMDPIde_CH
dc.relation.ispartofCellsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectDrug testingde_CH
dc.subjectExtracellular matrixde_CH
dc.subjectHuman iPSC derived astrocyte and dopaminergic neuronsde_CH
dc.subjectMacromolecular crowdingde_CH
dc.subjectCell differentiationde_CH
dc.subjectCoculture techniquede_CH
dc.subjectHumansde_CH
dc.subjectMacromolecular substancede_CH
dc.subjectCell culture techniquede_CH
dc.subjectExtracellular matrixde_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.titleEnhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowdingde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.3390/cells11142131de_CH
dc.identifier.doi10.21256/zhaw-26839-
dc.identifier.pmid35883574de_CH
zhaw.funding.euNode_CH
zhaw.issue14de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start2131de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume11de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Vo, A. N., Kundu, S., Strong, C., Jung, O., Lee, E., Song, M. J., Boutin, M. E., Raghunath, M., & Ferrer, M. (2022). Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding. Cells, 11(14), 2131. https://doi.org/10.3390/cells11142131
Vo, A.N. et al. (2022) ‘Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding’, Cells, 11(14), p. 2131. Available at: https://doi.org/10.3390/cells11142131.
A. N. Vo et al., “Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding,” Cells, vol. 11, no. 14, p. 2131, 2022, doi: 10.3390/cells11142131.
VO, Andy N., Srikanya KUNDU, Caroline STRONG, Olive JUNG, Emily LEE, Min Jae SONG, Molly E. BOUTIN, Michael RAGHUNATH und Marc FERRER, 2022. Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding. Cells. 2022. Bd. 11, Nr. 14, S. 2131. DOI 10.3390/cells11142131
Vo, Andy N., Srikanya Kundu, Caroline Strong, Olive Jung, Emily Lee, Min Jae Song, Molly E. Boutin, Michael Raghunath, and Marc Ferrer. 2022. “Enhancement of Neuroglial Extracellular Matrix Formation and Physiological Activity of Dopaminergic Neural Cocultures by Macromolecular Crowding.” Cells 11 (14): 2131. https://doi.org/10.3390/cells11142131.
Vo, Andy N., et al. “Enhancement of Neuroglial Extracellular Matrix Formation and Physiological Activity of Dopaminergic Neural Cocultures by Macromolecular Crowding.” Cells, vol. 11, no. 14, 2022, p. 2131, https://doi.org/10.3390/cells11142131.


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