|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica|
|Published in:||European Journal of Plant Pathology|
|Publisher / Ed. Institution:||Springer|
|Subjects:||Chestnut blight; Genetic diversity; Diagnostics; Biocontrol; Plant pathogen; Ascomycete|
|Subject (DDC):||571: Physiology and related subjects |
|Abstract:||The highly destructive chestnut blight disease can be successfully controlled by infecting the virulent strain of C. parasitica with the hyperparasitic mycovirus CHV-1. The artificial application of the virus-induced hypovirulence, however, requires that the vegetative compatibility (vc) diversity in the target C. parasitica population be determined beforehand. Conventional vc type determination by pairing the unknown isolate with tester strains of known vc types is time consuming and not always reliable. A genotyping assay was developed that uses DNA from C. parasitica isolates and discriminates the six known di-allelic vic loci and the two mating idiomorphs in two fluorescence-labeled multiplex PCRs. Validation was conducted by successfully genotyping the 74 known European vc tester strains and a conventionally characterized natural population of C. parasitica. Cross-species amplification tests with three Cryphonectria species (C. japonica, C. naterciae, and C. radicalis) revealed a high amplification specificity for C. parasitica, while the analytical sensitivity of the assay was established at 20 pg per reaction of genomic DNA. In conclusion, this fluorescent multiplex genotyping assay offers a simple and high-throughput tool for the characterization of the vegetative compatibility and mating type of C. parasitica at population level, which is particularly important for the application of mycovirus-mediated biological control of chestnut blight.|
|Further description:||Erworben im Rahmen der Schweizer Nationallizenzen (http://www.nationallizenzen.ch)|
|Fulltext version:||Published version|
|License (according to publishing contract):||Licence according to publishing contract|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Appears in collections:||Publikationen Life Sciences und Facility Management|
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Cornejo, C., Šever, B., Kupper, Q., Prospero, S., & Rigling, D. (2019). A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica. European Journal of Plant Pathology, 155(1), 81–91. https://doi.org/10.1007/s10658-019-01751-w
Cornejo, C. et al. (2019) ‘A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica’, European Journal of Plant Pathology, 155(1), pp. 81–91. Available at: https://doi.org/10.1007/s10658-019-01751-w.
C. Cornejo, B. Šever, Q. Kupper, S. Prospero, and D. Rigling, “A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica,” European Journal of Plant Pathology, vol. 155, no. 1, pp. 81–91, 2019, doi: 10.1007/s10658-019-01751-w.
Cornejo, C., et al. “A Multiplexed Genotyping Assay to Determine Vegetative Incompatibility and Mating Type in Cryphonectria Parasitica.” European Journal of Plant Pathology, vol. 155, no. 1, 2019, pp. 81–91, https://doi.org/10.1007/s10658-019-01751-w.
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