| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica |
| Autor/-in: | Cornejo, C. Šever, B. Kupper, Q. Prospero, S. Rigling, D. |
| et. al: | No |
| DOI: | 10.1007/s10658-019-01751-w |
| Erschienen in: | European Journal of Plant Pathology |
| Band(Heft): | 155 |
| Heft: | 1 |
| Seite(n): | 81 |
| Seiten bis: | 91 |
| Erscheinungsdatum: | 2019 |
| Verlag / Hrsg. Institution: | Springer |
| ISSN: | 0929-1873 1573-8469 |
| Sprache: | Englisch |
| Schlagwörter: | Chestnut blight; Genetic diversity; Diagnostics; Biocontrol; Plant pathogen; Ascomycete |
| Fachgebiet (DDC): | 571: Physiologie und verwandte Themen 572: Biochemie |
| Zusammenfassung: | The highly destructive chestnut blight disease can be successfully controlled by infecting the virulent strain of C. parasitica with the hyperparasitic mycovirus CHV-1. The artificial application of the virus-induced hypovirulence, however, requires that the vegetative compatibility (vc) diversity in the target C. parasitica population be determined beforehand. Conventional vc type determination by pairing the unknown isolate with tester strains of known vc types is time consuming and not always reliable. A genotyping assay was developed that uses DNA from C. parasitica isolates and discriminates the six known di-allelic vic loci and the two mating idiomorphs in two fluorescence-labeled multiplex PCRs. Validation was conducted by successfully genotyping the 74 known European vc tester strains and a conventionally characterized natural population of C. parasitica. Cross-species amplification tests with three Cryphonectria species (C. japonica, C. naterciae, and C. radicalis) revealed a high amplification specificity for C. parasitica, while the analytical sensitivity of the assay was established at 20 pg per reaction of genomic DNA. In conclusion, this fluorescent multiplex genotyping assay offers a simple and high-throughput tool for the characterization of the vegetative compatibility and mating type of C. parasitica at population level, which is particularly important for the application of mycovirus-mediated biological control of chestnut blight. |
| Weitere Angaben: | Erworben im Rahmen der Schweizer Nationallizenzen (http://www.nationallizenzen.ch) |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/26738 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
Es gibt keine Dateien zu dieser Ressource.
Zur Langanzeige
Cornejo, C., Šever, B., Kupper, Q., Prospero, S., & Rigling, D. (2019). A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica. European Journal of Plant Pathology, 155(1), 81–91. https://doi.org/10.1007/s10658-019-01751-w
Cornejo, C. et al. (2019) ‘A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica’, European Journal of Plant Pathology, 155(1), pp. 81–91. Available at: https://doi.org/10.1007/s10658-019-01751-w.
C. Cornejo, B. Šever, Q. Kupper, S. Prospero, and D. Rigling, “A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica,” European Journal of Plant Pathology, vol. 155, no. 1, pp. 81–91, 2019, doi: 10.1007/s10658-019-01751-w.
CORNEJO, C., B. ŠEVER, Q. KUPPER, S. PROSPERO und D. RIGLING, 2019. A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica. European Journal of Plant Pathology. 2019. Bd. 155, Nr. 1, S. 81–91. DOI 10.1007/s10658-019-01751-w
Cornejo, C., B. Šever, Q. Kupper, S. Prospero, and D. Rigling. 2019. “A Multiplexed Genotyping Assay to Determine Vegetative Incompatibility and Mating Type in Cryphonectria Parasitica.” European Journal of Plant Pathology 155 (1): 81–91. https://doi.org/10.1007/s10658-019-01751-w.
Cornejo, C., et al. “A Multiplexed Genotyping Assay to Determine Vegetative Incompatibility and Mating Type in Cryphonectria Parasitica.” European Journal of Plant Pathology, vol. 155, no. 1, 2019, pp. 81–91, https://doi.org/10.1007/s10658-019-01751-w.
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt, soweit nicht anderweitig angezeigt.