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Title: Incorporation of a FRET dye pair into mesoporous materials : a comparison of fluorescence spectra, FRET activity and dye accessibility
Authors : Widmer, Susanne
Reber, Michael J.
Müller, Patrick
Housecroft, Catherine E.
Constable, Edwin C.
Rossi, René M.
Brühwiler, Dominik
Scherer, Lukas J.
Boesel, Luciano F.
Published in : The Analyst
Volume(Issue) : 140
Issue : 15
Pages : 5324
Pages to: 5334
Publisher / Ed. Institution : Royal Society of Chemistry
Issue Date: 2015
Language : Englisch / English
Subjects : Sensor; Mesopores; SBA-15; Ammonia
Subject (DDC) : 540: Chemie
Abstract: Fluorescein and rhodamine B modified mesoporous silica particles were synthesized by post-grafting and co-condensation approaches. The materials exhibited different pore size distributions, particle shapes and sizes. The materials were characterized by nitrogen sorption, scanning electron microscopy and fluorescence spectroscopy. The Förster resonance energy transfer between the selected dye pair was explored for the different materials by exposure to various concentrations of gaseous ammonia. A logarithmic increase in rhodamine B emission with increasing ammonia concentration was observed for both post-grafted and co-condensed materials. The dye accessibility by ammonia gas in the silica framework of mesoporous materials was evaluated by using a flow cell gas sensor setup built in-house. Response to ammonia gas and recovery with nitrogen gas are explained by comparing the structure properties and dye loading of the materials. The post-grafted dye modified silica showed better performance in terms of reversibility and recovery.
Further description : Open Access Artikel
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Chemie und Biotechnologie (ICBT)
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
Type of review: Peer review (Publikation)
DOI : 10.1039/C5AN00850F
ISSN: 0003-2654
License (according to publishing contract) : CC BY 3.0: Namensnennung 3.0 Unported
Published as part of the ZHAW project : Molecular Alignment Chips (MACs)
Appears in Collections:Publikationen Life Sciences und Facility Management

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