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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Effect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitats
Authors: Joshi, Akshay
Young, Diana
Huang, Liren
Mosberger, Lona
Munk, Bernhard
Vinzelj, Julia
Flad, Veronika
Sczyrba, Alexander
Griffith, Gareth W.
Podmirseg, Sabine Marie
Warthmann, Rolf
Lebuhn, Michael
Insam, Heribert
et. al: No
DOI: 10.3390/microorganisms10101972
Published in: Microorganisms
Volume(Issue): 10
Issue: 10
Page(s): 1972
Issue Date: Oct-2022
Publisher / Ed. Institution: MDPI
ISSN: 2076-2607
Language: English
Subjects: Microbiology; Anaerobic fungus; Neocallimastigomycota; Ruminant; Hindgut animal; Minimal medium; Novel genus; Selective isolation; Enrichment
Subject (DDC): 579: Microbiology
Abstract: Anaerobic fungi (AF), belonging to the phylum Neocallimastigomycota, are a pivotal component of the digestive tract microbiome of various herbivorous animals. In the last decade, the diversity of AF has rapidly expanded due to the exploration of numerous (novel) habitats. Studies aiming at understanding the role of AF require robust and reliable isolation and cultivation techniques, many of which remained unchanged for decades. Using amplicon sequencing, we compared three different media: medium with rumen fluid (RF), depleted rumen fluid (DRF), and no rumen fluid (NRF) to enrich the AF from the feces of yak, as a rumen control; and Przewalski’s horse, llama, guanaco, and elephant, as a non-rumen habitats. The results revealed the selective enrichment of Piromyces and Neocallimastix from the feces of elephant and llama, respectively, in the RF medium. Similarly, the enrichment culture in DRF medium explicitly manifested Piromyces-related sequences from elephant feces. Five new clades (MM1-5) were defined from llama, guanaco, yak, and elephant feces that could as well be enriched from llama and elephant samples using non-conventional DRF and NRF media. This study presents evidence for the selective enrichment of certain genera in medium with RF and DRF from rumen as well as from non-rumen samples. NRF medium is suggested for the isolation of AF from non-rumen environments.
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Published as part of the ZHAW project: Entfesselung des versteckten Potentials anaerober Pilze (Neocallimastigomycota)
Appears in collections:Publikationen Life Sciences und Facility Management

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