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https://doi.org/10.21256/zhaw-25803| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Effect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitats |
| Autor/-in: | Joshi, Akshay Young, Diana Huang, Liren Mosberger, Lona Munk, Bernhard Vinzelj, Julia Flad, Veronika Sczyrba, Alexander Griffith, Gareth W. Podmirseg, Sabine Marie Warthmann, Rolf Lebuhn, Michael Insam, Heribert |
| et. al: | No |
| DOI: | 10.3390/microorganisms10101972 10.21256/zhaw-25803 |
| Erschienen in: | Microorganisms |
| Band(Heft): | 10 |
| Heft: | 10 |
| Seite(n): | 1972 |
| Erscheinungsdatum: | Okt-2022 |
| Verlag / Hrsg. Institution: | MDPI |
| ISSN: | 2076-2607 |
| Sprache: | Englisch |
| Schlagwörter: | Microbiology; Anaerobic fungus; Neocallimastigomycota; Ruminant; Hindgut animal; Minimal medium; Novel genus; Selective isolation; Enrichment |
| Fachgebiet (DDC): | 579: Mikrobiologie |
| Zusammenfassung: | Anaerobic fungi (AF), belonging to the phylum Neocallimastigomycota, are a pivotal component of the digestive tract microbiome of various herbivorous animals. In the last decade, the diversity of AF has rapidly expanded due to the exploration of numerous (novel) habitats. Studies aiming at understanding the role of AF require robust and reliable isolation and cultivation techniques, many of which remained unchanged for decades. Using amplicon sequencing, we compared three different media: medium with rumen fluid (RF), depleted rumen fluid (DRF), and no rumen fluid (NRF) to enrich the AF from the feces of yak, as a rumen control; and Przewalski’s horse, llama, guanaco, and elephant, as a non-rumen habitats. The results revealed the selective enrichment of Piromyces and Neocallimastix from the feces of elephant and llama, respectively, in the RF medium. Similarly, the enrichment culture in DRF medium explicitly manifested Piromyces-related sequences from elephant feces. Five new clades (MM1-5) were defined from llama, guanaco, yak, and elephant feces that could as well be enriched from llama and elephant samples using non-conventional DRF and NRF media. This study presents evidence for the selective enrichment of certain genera in medium with RF and DRF from rumen as well as from non-rumen samples. NRF medium is suggested for the isolation of AF from non-rumen environments. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/25803 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY 4.0: Namensnennung 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Publiziert im Rahmen des ZHAW-Projekts: | Entfesselung des versteckten Potentials anaerober Pilze (Neocallimastigomycota) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2022_Joshi-etal_Growth-media-Neocallimastigomycetes-diversity.pdf | 854.59 kB | Adobe PDF |  Öffnen/Anzeigen |
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Joshi, A., Young, D., Huang, L., Mosberger, L., Munk, B., Vinzelj, J., Flad, V., Sczyrba, A., Griffith, G. W., Podmirseg, S. M., Warthmann, R., Lebuhn, M., & Insam, H. (2022). Effect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitats. Microorganisms, 10(10), 1972. https://doi.org/10.3390/microorganisms10101972
Joshi, A. et al. (2022) ‘Effect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitats’, Microorganisms, 10(10), p. 1972. Available at: https://doi.org/10.3390/microorganisms10101972.
A. Joshi et al., “Effect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitats,” Microorganisms, vol. 10, no. 10, p. 1972, Oct. 2022, doi: 10.3390/microorganisms10101972.
JOSHI, Akshay, Diana YOUNG, Liren HUANG, Lona MOSBERGER, Bernhard MUNK, Julia VINZELJ, Veronika FLAD, Alexander SCZYRBA, Gareth W. GRIFFITH, Sabine Marie PODMIRSEG, Rolf WARTHMANN, Michael LEBUHN und Heribert INSAM, 2022. Effect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitats. Microorganisms. Oktober 2022. Bd. 10, Nr. 10, S. 1972. DOI 10.3390/microorganisms10101972
Joshi, Akshay, Diana Young, Liren Huang, Lona Mosberger, Bernhard Munk, Julia Vinzelj, Veronika Flad, et al. 2022. “Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats.” Microorganisms 10 (10): 1972. https://doi.org/10.3390/microorganisms10101972.
Joshi, Akshay, et al. “Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats.” Microorganisms, vol. 10, no. 10, Oct. 2022, p. 1972, https://doi.org/10.3390/microorganisms10101972.
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