Title: Engineering characteristics of a single-use stirred bioreactor at benchtop scale : the Mobius CellReady 3 L bioreactor as case study
Authors : Kaiser, Stephan
Eibl-Schindler, Regine
Eibl, Dieter
Published in : Engineering in Life Sciences
Volume(Issue) : 11
Issue : 4
Pages : 359
Pages to: 368
Publisher / Ed. Institution : Wiley
Publisher / Ed. Institution: Weinheim
Issue Date: Aug-2011
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subject (DDC) : 660: Chemical engineering
Abstract: Stirred single-use bioreactors can be used as substitutes for their conventional counterparts made of glass or stainless steel in the development and production of biopharmaceuticals wherever possible. Various studies have confirmed their comparability in cell growth as well as in product quantity and quality. However, information about their engineering characteristics is still rare. This study focuses on the stirred Mobius® CellReady 3L bioreactor. The main engineering parameters for typical operation conditions used in animal cell cultivations are presented for the first time. Numerical simulations with a commercial CFD package (Fluent 6.3) were accomplished to obtain data on the single- and multi-phase fluid flow, power input, mixing time and oxygen mass transfer. The results, which were compared with data from experiments and from the literature, reveal the suitability of the Mobius® CellReady 3L bioreactor for cell expansion and protein production with animal cell cultures. Furthermore, the data enable comparisons with other single-use and reusable cell culture bioreactors at bench-scale.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific journal
DOI : 10.1002/elsc.201000171
ISSN: 1618-0240
URI: https://digitalcollection.zhaw.ch/handle/11475/2318
Appears in Collections:Publikationen Life Sciences und Facility Management

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