Title: Fluid flow and cell proliferation of mesenchymal adipose-derived stem cells in small-scale, stirred, single-use bioreactors
Authors : Kaiser, Stephan
Jossen, Valentin
Schirmaier, Carmen
Eibl, Dieter
Brill, Silke
van den Bos, Christian
Eibl-Schindler, Regine
Published in : Chemie Ingenieur Technik
Volume(Issue) : 85
Issue : 1-2
Pages : 95
Pages to: 102
Publisher / Ed. Institution : Wiley
Publisher / Ed. Institution: Weinheim
Issue Date: Feb-2013
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subject (DDC) : 660: Chemical engineering
Abstract: The fluid flow and suspension characteristics inside small-scale, stirred, single-use bioreactors were investigated experimentally and by means of computational fluid dynamics. The required impeller speeds for homogenous suspension were determined for two microcarrier types. The shear stress level and turbulence distribution were predicted using a numerical model, which was verified by particle image velocimetry measurements. In subsequent cultivations of primary mesenchymal adipose-derived stem cells, up to 31.4-fold expansion in cell number was achieved for serum concentrations as low as 5 %.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific journal
DOI : 10.1002/cite.201200180
ISSN: 0009-286X
URI: https://digitalcollection.zhaw.ch/handle/11475/2239
Appears in Collections:Publikationen Life Sciences und Facility Management

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