Fluid flow and cell proliferation of mesenchymal adipose-derived stem cells in small-scale, stirred, single-use bioreactors

Kaiser, Stephan; Jossen, Valentin; Schirmaier, Carmen; Eibl, Dieter; Brill, Silke; van den Bos, Christian; Eibl-Schindler, Regine

doi:10.1002/cite.201200180

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Fluid flow and cell proliferation of mesenchymal adipose-derived stem cells in small-scale, stirred, single-use bioreactors
Authors:	Kaiser, Stephan Jossen, Valentin Schirmaier, Carmen Eibl, Dieter Brill, Silke van den Bos, Christian Eibl-Schindler, Regine
DOI:	10.1002/cite.201200180
Published in:	Chemie Ingenieur Technik
Volume(Issue):	85
Issue:	1-2
Page(s):	95
Pages to:	102
Issue Date:	Feb-2013
Publisher / Ed. Institution:	Wiley
Publisher / Ed. Institution:	Weinheim
ISSN:	0009-286X 1522-2640
Language:	English
Subject (DDC):	660: Chemical engineering
Abstract:	The fluid flow and suspension characteristics inside small-scale, stirred, single-use bioreactors were investigated experimentally and by means of computational fluid dynamics. The required impeller speeds for homogenous suspension were determined for two microcarrier types. The shear stress level and turbulence distribution were predicted using a numerical model, which was verified by particle image velocimetry measurements. In subsequent cultivations of primary mesenchymal adipose-derived stem cells, up to 31.4-fold expansion in cell number was achieved for serum concentrations as low as 5 %.
URI:	https://digitalcollection.zhaw.ch/handle/11475/2239
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

Files in This Item:

There are no files associated with this item.

Show full item record

Kaiser, S., Jossen, V., Schirmaier, C., Eibl, D., Brill, S., van den Bos, C., & Eibl-Schindler, R. (2013). Fluid flow and cell proliferation of mesenchymal adipose-derived stem cells in small-scale, stirred, single-use bioreactors. Chemie Ingenieur Technik, 85(1-2), 95–102. https://doi.org/10.1002/cite.201200180

Kaiser, S. et al. (2013) ‘Fluid flow and cell proliferation of mesenchymal adipose-derived stem cells in small-scale, stirred, single-use bioreactors’, Chemie Ingenieur Technik, 85(1-2), pp. 95–102. Available at: https://doi.org/10.1002/cite.201200180.

S. Kaiser et al., “Fluid flow and cell proliferation of mesenchymal adipose-derived stem cells in small-scale, stirred, single-use bioreactors,” Chemie Ingenieur Technik, vol. 85, no. 1-2, pp. 95–102, Feb. 2013, doi: 10.1002/cite.201200180.

KAISER, Stephan, Valentin JOSSEN, Carmen SCHIRMAIER, Dieter EIBL, Silke BRILL, Christian VAN DEN BOS und Regine EIBL-SCHINDLER, 2013. Fluid flow and cell proliferation of mesenchymal adipose-derived stem cells in small-scale, stirred, single-use bioreactors. Chemie Ingenieur Technik. Februar 2013. Bd. 85, Nr. 1-2, S. 95–102. DOI 10.1002/cite.201200180

Kaiser, Stephan, Valentin Jossen, Carmen Schirmaier, Dieter Eibl, Silke Brill, Christian van den Bos, and Regine Eibl-Schindler. 2013. “Fluid Flow and Cell Proliferation of Mesenchymal Adipose-Derived Stem Cells in Small-Scale, Stirred, Single-Use Bioreactors.” Chemie Ingenieur Technik 85 (1-2): 95–102. https://doi.org/10.1002/cite.201200180.

Kaiser, Stephan, et al. “Fluid Flow and Cell Proliferation of Mesenchymal Adipose-Derived Stem Cells in Small-Scale, Stirred, Single-Use Bioreactors.” Chemie Ingenieur Technik, vol. 85, no. 1-2, Feb. 2013, pp. 95–102, https://doi.org/10.1002/cite.201200180.