Verbascum nigrum : cytotoxicity evaluation in A431 epidermoid carcinoma cells and untargeted LC‐HR‐MS/MS metabolite profiling

Iliescu, Iolanda Alca; Peter, Samuel; Albert, Ina; Skalicka‐Woźniak, Krystyna; Miron, Anca; Luca, Simon Vlad; Wolfram, Evelyn

doi:10.1002/cbdv.202000644

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-22287

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Verbascum nigrum : cytotoxicity evaluation in A431 epidermoid carcinoma cells and untargeted LC‐HR‐MS/MS metabolite profiling
Authors:	Iliescu, Iolanda Alca Peter, Samuel Albert, Ina Skalicka‐Woźniak, Krystyna Miron, Anca Luca, Simon Vlad Wolfram, Evelyn
et. al:	No
DOI:	10.1002/cbdv.202000644 10.21256/zhaw-22287
Published in:	Chemistry & Biodiversity
Volume(Issue):	17
Issue:	12
Page(s):	e2000644
Issue Date:	2020
Publisher / Ed. Institution:	Wiley
ISSN:	1612-1872 1612-1880
Language:	English
Subjects:	LC/HR-MS/MS; Verbascum nigrum; Cytotoxic activity; Saponin glycosides; Verbascoside
Subject (DDC):	572: Biochemistry
Abstract:	The crude methanolic extract obtained from Verbascum nigrum aerial parts (VNE) and its six fractions (VNF1-VNF6) were initially screened regarding their effects on the viability of immortalized HaCaT keratinocytes and A431 epidermoid carcinoma cells (MTT assay, 24 h). None of the tested samples affected the viability of HaCaT cells in a concentration range of 25-150 μg/mL. VNE and VNF4 exhibited significant cytotoxic effects in A431 cells, with IC50 values of 81.92 and 12.27 μg/mL, respectively; the selectivity index was higher than 10 for VNF4. The untargeted LC/HR-MS/MS metabolite profiling led to the tentative annotation of a total number of 23 compounds. Of these, VNE comprised mainly iridoid glycosides (harpagoside, laterioside, acylated aucubin derivatives), whereas VNF4 showed a high abundance of triterpene saponin glycosides (ilwensisaponins A and C, songarosaponins A and B), constituents known for their selective cytotoxic potential.
URI:	https://digitalcollection.zhaw.ch/handle/11475/22287
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Iliescu, I. A., Peter, S., Albert, I., Skalicka‐Woźniak, K., Miron, A., Luca, S. V., & Wolfram, E. (2020). Verbascum nigrum : cytotoxicity evaluation in A431 epidermoid carcinoma cells and untargeted LC‐HR‐MS/MS metabolite profiling. Chemistry & Biodiversity, 17(12), e2000644. https://doi.org/10.1002/cbdv.202000644

Iliescu, I.A. et al. (2020) ‘Verbascum nigrum : cytotoxicity evaluation in A431 epidermoid carcinoma cells and untargeted LC‐HR‐MS/MS metabolite profiling’, Chemistry & Biodiversity, 17(12), p. e2000644. Available at: https://doi.org/10.1002/cbdv.202000644.

I. A. Iliescu et al., “Verbascum nigrum : cytotoxicity evaluation in A431 epidermoid carcinoma cells and untargeted LC‐HR‐MS/MS metabolite profiling,” Chemistry & Biodiversity, vol. 17, no. 12, p. e2000644, 2020, doi: 10.1002/cbdv.202000644.

ILIESCU, Iolanda Alca, Samuel PETER, Ina ALBERT, Krystyna SKALICKA‐WOŹNIAK, Anca MIRON, Simon Vlad LUCA und Evelyn WOLFRAM, 2020. Verbascum nigrum : cytotoxicity evaluation in A431 epidermoid carcinoma cells and untargeted LC‐HR‐MS/MS metabolite profiling. Chemistry & Biodiversity. 2020. Bd. 17, Nr. 12, S. e2000644. DOI 10.1002/cbdv.202000644

Iliescu, Iolanda Alca, Samuel Peter, Ina Albert, Krystyna Skalicka‐Woźniak, Anca Miron, Simon Vlad Luca, and Evelyn Wolfram. 2020. “Verbascum Nigrum : Cytotoxicity Evaluation in A431 Epidermoid Carcinoma Cells and Untargeted LC‐HR‐MS/MS Metabolite Profiling.” Chemistry & Biodiversity 17 (12): e2000644. https://doi.org/10.1002/cbdv.202000644.

Iliescu, Iolanda Alca, et al. “Verbascum Nigrum : Cytotoxicity Evaluation in A431 Epidermoid Carcinoma Cells and Untargeted LC‐HR‐MS/MS Metabolite Profiling.” Chemistry & Biodiversity, vol. 17, no. 12, 2020, p. e2000644, https://doi.org/10.1002/cbdv.202000644.