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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Verbascum nigrum : cytotoxicity evaluation in A431 epidermoid carcinoma cells and untargeted LC‐HR‐MS/MS metabolite profiling
Authors: Iliescu, Iolanda Alca
Peter, Samuel
Albert, Ina
Skalicka‐Woźniak, Krystyna
Miron, Anca
Luca, Simon Vlad
Wolfram, Evelyn
et. al: No
DOI: 10.1002/cbdv.202000644
Published in: Chemistry & Biodiversity
Volume(Issue): 17
Issue: 12
Page(s): e2000644
Issue Date: 2020
Publisher / Ed. Institution: Wiley
ISSN: 1612-1872
Language: English
Subjects: LC/HR-MS/MS; Verbascum nigrum; Cytotoxic activity; Saponin glycosides; Verbascoside
Subject (DDC): 572: Biochemistry
Abstract: The crude methanolic extract obtained from Verbascum nigrum aerial parts (VNE) and its six fractions (VNF1-VNF6) were initially screened regarding their effects on the viability of immortalized HaCaT keratinocytes and A431 epidermoid carcinoma cells (MTT assay, 24 h). None of the tested samples affected the viability of HaCaT cells in a concentration range of 25-150 μg/mL. VNE and VNF4 exhibited significant cytotoxic effects in A431 cells, with IC50 values of 81.92 and 12.27 μg/mL, respectively; the selectivity index was higher than 10 for VNF4. The untargeted LC/HR-MS/MS metabolite profiling led to the tentative annotation of a total number of 23 compounds. Of these, VNE comprised mainly iridoid glycosides (harpagoside, laterioside, acylated aucubin derivatives), whereas VNF4 showed a high abundance of triterpene saponin glycosides (ilwensisaponins A and C, songarosaponins A and B), constituents known for their selective cytotoxic potential.
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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