Permanent hydrophilization and generic bioactivation of melt electrowritten scaffolds

Bertlein, Sarah; Hochleitner, Gernot; Schmitz, Michael; Tessmar, Jörg; Raghunath, Michael; Dalton, Paul D.; Groll, Jürgen

doi:10.1002/adhm.201801544

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Permanent hydrophilization and generic bioactivation of melt electrowritten scaffolds
Authors:	Bertlein, Sarah Hochleitner, Gernot Schmitz, Michael Tessmar, Jörg Raghunath, Michael Dalton, Paul D. Groll, Jürgen
et. al:	No
DOI:	10.1002/adhm.201801544
Published in:	Advanced Healthcare Materials
Volume(Issue):	8
Issue:	7
Issue Date:	11-Apr-2019
Publisher / Ed. Institution:	Wiley
ISSN:	2192-2640 2192-2659
Language:	English
Subjects:	PCL; Biofunctionalization; Melt electrowriting; Scaffolds; Surface modification; Cell adhesion; Collagen; Human; Hydrogel; Hydrophobic and hydrophilic interactions; Leucine; Lysine; Mesenchymal stem cell; Oligopeptides; Polyester; Polyethylenes; Polypropylenes; Tissue scaffolds; Ultraviolet rays
Subject (DDC):	610.28: Biomedicine, biomedical engineering
Abstract:	Melt electrowriting (MEW) is an emerging additive manufacturing technology that direct-writes low-micron diameter fibers into 3D scaffolds with high porosities. Often, the polymers currently used for MEW are hydrophobic thermoplastics that induce unspecific protein adsorption and subsequent uncontrolled cell adhesion. Here are developed a coating strategy for MEW scaffolds based on six-arm star-shaped NCO-poly(ethylene oxide-stat-propylene oxide) (sP(EO-stat-PO)). This permanently hydrophilizes the PCL through the formation of a hydrogel coating and minimizes unspecific interactions with proteins and cells. It also provides the option of simultaneous covalent attachment of bioactive molecules through reaction with isocyanates before these are hydrolyzed. Furthermore, a photoactivatable chemical functionalization is introduced that is not dependent on the time-limited window of isocyanate chemistry. For this, photo-leucine is covalently immobilized into the sP(EO-stat-PO) layer, resulting in a photoactivatable scaffold that enables the binding of sterically demanding molecules at any timepoint after scaffold preparation and coating and is decoupled from the isocyanate chemistry. A successful biofunctionalization of MEW scaffolds via this strategy is demonstrated with streptavidin and collagen as examples. This hydrogel coating system is a generic one that introduces flexible specific and multiple surface functionalization, potentially for a spectrum of polymers made from different manufacturing processes.
URI:	https://digitalcollection.zhaw.ch/handle/11475/22183
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Bertlein, S., Hochleitner, G., Schmitz, M., Tessmar, J., Raghunath, M., Dalton, P. D., & Groll, J. (2019). Permanent hydrophilization and generic bioactivation of melt electrowritten scaffolds. Advanced Healthcare Materials, 8(7). https://doi.org/10.1002/adhm.201801544

Bertlein, S. et al. (2019) ‘Permanent hydrophilization and generic bioactivation of melt electrowritten scaffolds’, Advanced Healthcare Materials, 8(7). Available at: https://doi.org/10.1002/adhm.201801544.

S. Bertlein et al., “Permanent hydrophilization and generic bioactivation of melt electrowritten scaffolds,” Advanced Healthcare Materials, vol. 8, no. 7, Apr. 2019, doi: 10.1002/adhm.201801544.

BERTLEIN, Sarah, Gernot HOCHLEITNER, Michael SCHMITZ, Jörg TESSMAR, Michael RAGHUNATH, Paul D. DALTON und Jürgen GROLL, 2019. Permanent hydrophilization and generic bioactivation of melt electrowritten scaffolds. Advanced Healthcare Materials. 11 April 2019. Bd. 8, Nr. 7. DOI 10.1002/adhm.201801544

Bertlein, Sarah, Gernot Hochleitner, Michael Schmitz, Jörg Tessmar, Michael Raghunath, Paul D. Dalton, and Jürgen Groll. 2019. “Permanent Hydrophilization and Generic Bioactivation of Melt Electrowritten Scaffolds.” Advanced Healthcare Materials 8 (7). https://doi.org/10.1002/adhm.201801544.

Bertlein, Sarah, et al. “Permanent Hydrophilization and Generic Bioactivation of Melt Electrowritten Scaffolds.” Advanced Healthcare Materials, vol. 8, no. 7, Apr. 2019, https://doi.org/10.1002/adhm.201801544.