| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway |
| Autor/-in: | Ritala, Anneli Dong, Lemeng Imseng, Nicole Seppänen-Laakso, Tuulikki Vasilev, Nikolay van der Krol, Sander Rischer, Heiko Maaheimo, Hannu Virkki, Arho Brändli, Johanna Schillberg, Stefan Eibl-Schindler, Regine Bouwmeester, Harro Oksman-Caldentey, Kirsi-Marja |
| DOI: | 10.1016/j.jbiotec.2014.01.031 |
| Erschienen in: | Journal of Biotechnology |
| Band(Heft): | 176 |
| Seite(n): | 20 |
| Seiten bis: | 28 |
| Erscheinungsdatum: | 2014 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 0168-1656 1873-4863 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 660.6: Biotechnologie |
| Zusammenfassung: | The terpenoid indole alkaloids are one of the major classes of plant-derived natural products and are well known for their many applications in the pharmaceutical, fragrance and cosmetics industries. Hairy root cultures are useful for the production of plant secondary metabolites because of their genetic and biochemical stability and their rapid growth in hormone-free media. Tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots, which do not produce geraniol naturally, were engineered to express a plastid-targeted geraniol synthase gene originally isolated from Valeriana officinalis L. (VoGES). A SPME-GC-MS screening tool was developed for the rapid evaluation of production clones. The GC-MS analysis revealed that the free geraniol content in 20 hairy root clones expressing VoGES was an average of 13.7μg/g dry weight (DW) and a maximum of 31.3μg/g DW. More detailed metabolic analysis revealed that geraniol derivatives were present in six major glycoside forms, namely the hexose and/or pentose conjugates of geraniol and hydroxygeraniol, resulting in total geraniol levels of up to 204.3μg/g DW following deglycosylation. A benchtop-scale process was developed in a 20-L wave-mixed bioreactor eventually yielding hundreds of grams of biomass and milligram quantities of geraniol per cultivation bag. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/2077 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Ritala, A., Dong, L., Imseng, N., Seppänen-Laakso, T., Vasilev, N., van der Krol, S., Rischer, H., Maaheimo, H., Virkki, A., Brändli, J., Schillberg, S., Eibl-Schindler, R., Bouwmeester, H., & Oksman-Caldentey, K.-M. (2014). Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway. Journal of Biotechnology, 176, 20–28. https://doi.org/10.1016/j.jbiotec.2014.01.031
Ritala, A. et al. (2014) ‘Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway’, Journal of Biotechnology, 176, pp. 20–28. Available at: https://doi.org/10.1016/j.jbiotec.2014.01.031.
A. Ritala et al., “Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway,” Journal of Biotechnology, vol. 176, pp. 20–28, 2014, doi: 10.1016/j.jbiotec.2014.01.031.
RITALA, Anneli, Lemeng DONG, Nicole IMSENG, Tuulikki SEPPÄNEN-LAAKSO, Nikolay VASILEV, Sander VAN DER KROL, Heiko RISCHER, Hannu MAAHEIMO, Arho VIRKKI, Johanna BRÄNDLI, Stefan SCHILLBERG, Regine EIBL-SCHINDLER, Harro BOUWMEESTER und Kirsi-Marja OKSMAN-CALDENTEY, 2014. Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway. Journal of Biotechnology. 2014. Bd. 176, S. 20–28. DOI 10.1016/j.jbiotec.2014.01.031
Ritala, Anneli, Lemeng Dong, Nicole Imseng, Tuulikki Seppänen-Laakso, Nikolay Vasilev, Sander van der Krol, Heiko Rischer, et al. 2014. “Evaluation of Tobacco (Nicotiana Tabacum L. Cv. Petit Havana SR1) Hairy Roots for the Production of Geraniol, the First Committed Step in Terpenoid Indole Alkaloid Pathway.” Journal of Biotechnology 176: 20–28. https://doi.org/10.1016/j.jbiotec.2014.01.031.
Ritala, Anneli, et al. “Evaluation of Tobacco (Nicotiana Tabacum L. Cv. Petit Havana SR1) Hairy Roots for the Production of Geraniol, the First Committed Step in Terpenoid Indole Alkaloid Pathway.” Journal of Biotechnology, vol. 176, 2014, pp. 20–28, https://doi.org/10.1016/j.jbiotec.2014.01.031.
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