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dc.contributor.authorRitala, Anneli-
dc.contributor.authorDong, Lemeng-
dc.contributor.authorImseng, Nicole-
dc.contributor.authorSeppänen-Laakso, Tuulikki-
dc.contributor.authorVasilev, Nikolay-
dc.contributor.authorvan der Krol, Sander-
dc.contributor.authorRischer, Heiko-
dc.contributor.authorMaaheimo, Hannu-
dc.contributor.authorVirkki, Arho-
dc.contributor.authorBrändli, Johanna-
dc.contributor.authorSchillberg, Stefan-
dc.contributor.authorEibl-Schindler, Regine-
dc.contributor.authorBouwmeester, Harro-
dc.contributor.authorOksman-Caldentey, Kirsi-Marja-
dc.date.accessioned2018-01-17T10:06:14Z-
dc.date.available2018-01-17T10:06:14Z-
dc.date.issued2014-
dc.identifier.issn0168-1656de_CH
dc.identifier.issn1873-4863de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/2077-
dc.description.abstractThe terpenoid indole alkaloids are one of the major classes of plant-derived natural products and are well known for their many applications in the pharmaceutical, fragrance and cosmetics industries. Hairy root cultures are useful for the production of plant secondary metabolites because of their genetic and biochemical stability and their rapid growth in hormone-free media. Tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots, which do not produce geraniol naturally, were engineered to express a plastid-targeted geraniol synthase gene originally isolated from Valeriana officinalis L. (VoGES). A SPME-GC-MS screening tool was developed for the rapid evaluation of production clones. The GC-MS analysis revealed that the free geraniol content in 20 hairy root clones expressing VoGES was an average of 13.7μg/g dry weight (DW) and a maximum of 31.3μg/g DW. More detailed metabolic analysis revealed that geraniol derivatives were present in six major glycoside forms, namely the hexose and/or pentose conjugates of geraniol and hydroxygeraniol, resulting in total geraniol levels of up to 204.3μg/g DW following deglycosylation. A benchtop-scale process was developed in a 20-L wave-mixed bioreactor eventually yielding hundreds of grams of biomass and milligram quantities of geraniol per cultivation bag.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofJournal of Biotechnologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleEvaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathwayde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.jbiotec.2014.01.031de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end28de_CH
zhaw.pages.start20de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume176de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Ritala, A., Dong, L., Imseng, N., Seppänen-Laakso, T., Vasilev, N., van der Krol, S., Rischer, H., Maaheimo, H., Virkki, A., Brändli, J., Schillberg, S., Eibl-Schindler, R., Bouwmeester, H., & Oksman-Caldentey, K.-M. (2014). Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway. Journal of Biotechnology, 176, 20–28. https://doi.org/10.1016/j.jbiotec.2014.01.031
Ritala, A. et al. (2014) ‘Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway’, Journal of Biotechnology, 176, pp. 20–28. Available at: https://doi.org/10.1016/j.jbiotec.2014.01.031.
A. Ritala et al., “Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway,” Journal of Biotechnology, vol. 176, pp. 20–28, 2014, doi: 10.1016/j.jbiotec.2014.01.031.
RITALA, Anneli, Lemeng DONG, Nicole IMSENG, Tuulikki SEPPÄNEN-LAAKSO, Nikolay VASILEV, Sander VAN DER KROL, Heiko RISCHER, Hannu MAAHEIMO, Arho VIRKKI, Johanna BRÄNDLI, Stefan SCHILLBERG, Regine EIBL-SCHINDLER, Harro BOUWMEESTER und Kirsi-Marja OKSMAN-CALDENTEY, 2014. Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway. Journal of Biotechnology. 2014. Bd. 176, S. 20–28. DOI 10.1016/j.jbiotec.2014.01.031
Ritala, Anneli, Lemeng Dong, Nicole Imseng, Tuulikki Seppänen-Laakso, Nikolay Vasilev, Sander van der Krol, Heiko Rischer, et al. 2014. “Evaluation of Tobacco (Nicotiana Tabacum L. Cv. Petit Havana SR1) Hairy Roots for the Production of Geraniol, the First Committed Step in Terpenoid Indole Alkaloid Pathway.” Journal of Biotechnology 176: 20–28. https://doi.org/10.1016/j.jbiotec.2014.01.031.
Ritala, Anneli, et al. “Evaluation of Tobacco (Nicotiana Tabacum L. Cv. Petit Havana SR1) Hairy Roots for the Production of Geraniol, the First Committed Step in Terpenoid Indole Alkaloid Pathway.” Journal of Biotechnology, vol. 176, 2014, pp. 20–28, https://doi.org/10.1016/j.jbiotec.2014.01.031.


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