Title: Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway
Authors : Ritala, Anneli
Dong, Lemeng
Imseng, Nicole
Seppänen-Laakso, Tuulikki
Vasilev, Nikolay
van der Krol, Sander
Rischer, Heiko
Maaheimo, Hannu
Virkki, Arho
Brändli, Johanna
Schillberg, Stefan
Eibl-Schindler, Regine
Bouwmeester, Harro
Oksman-Caldentey, Kirsi-Marja
Published in : Journal of Biotechnology
Volume(Issue) : 176
Pages : 20
Pages to: 28
Publisher / Ed. Institution : Elsevier BV
Issue Date: 2014
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 660.6: Biotechnology
Abstract: The terpenoid indole alkaloids are one of the major classes of plant-derived natural products and are well known for their many applications in the pharmaceutical, fragrance and cosmetics industries. Hairy root cultures are useful for the production of plant secondary metabolites because of their genetic and biochemical stability and their rapid growth in hormone-free media. Tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots, which do not produce geraniol naturally, were engineered to express a plastid-targeted geraniol synthase gene originally isolated from Valeriana officinalis L. (VoGES). A SPME-GC-MS screening tool was developed for the rapid evaluation of production clones. The GC-MS analysis revealed that the free geraniol content in 20 hairy root clones expressing VoGES was an average of 13.7μg/g dry weight (DW) and a maximum of 31.3μg/g DW. More detailed metabolic analysis revealed that geraniol derivatives were present in six major glycoside forms, namely the hexose and/or pentose conjugates of geraniol and hydroxygeraniol, resulting in total geraniol levels of up to 204.3μg/g DW following deglycosylation. A benchtop-scale process was developed in a 20-L wave-mixed bioreactor eventually yielding hundreds of grams of biomass and milligram quantities of geraniol per cultivation bag.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1016/j.jbiotec.2014.01.031
URI: https://digitalcollection.zhaw.ch/handle/11475/2077
Appears in Collections:Publikationen Life Sciences und Facility Management

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