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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS
Authors: Rothen, Julian
Sapugahawatte, Dulmini Nanayakkara
Li, Carmen
Lo, Norman
Vogel, Guido
Foucault, Frédéric
Pflüger, Valentin
Pothier, Joël
Blom, Jochen
Daubenberger, Claudia
Ip, Margaret
et. al: No
DOI: 10.1038/s41598-020-65707-5
Published in: Scientific Reports
Volume(Issue): 10
Issue: 8788
Issue Date: 29-May-2020
Publisher / Ed. Institution: Nature Publishing Group
ISSN: 2045-2322
Language: English
Subjects: Medical research; Microbiology
Subject (DDC): 570: Biology
Abstract: Streptococcus agalactiae (Group B Streptococcus, GBS), is a frequent human colonizer and a leading cause of neonatal meningitis as well as an emerging pathogen in non-pregnant adults. GBS possesses a broad animal host spectrum, and recent studies proved atypical GBS genotypes can cause human invasive diseases through animal sources as food-borne zoonotic infections. We applied a MALDI-TOF MS typing method, based on molecular weight variations of predefined 28 ribosomal subunit proteins (rsp) to classify GBS strains of varying serotypes into major phylogenetic lineages. A total of 249 GBS isolates of representative and varying capsular serotypes from patients and animal food sources (fish and pig) collected during 2016-2018 in Hong Kong were analysed. Over 84% (143/171) noninvasive carriage GBS strains from patients were readily typed into 5 globally dominant rsp-profiles. Among GBS strains from food animals, over 90% (57/63) of fish and 13% (2/15) of pig GBS matched with existing rsp-profiles, while the remainder were classified into two novel rsp-profiles and we failed to assign a fish strain into any cluster. MALDI-TOF MS allowed for high-throughput screening and simultaneous detection of novel, so far not well described GBS genotypes. The method shown here is rapid, simple, readily transferable and adapted for use in a diagnostic microbiology laboratory with potential for the surveillance of emerging GBS genotypes with zoonotic potential.
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project: MALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approaches
Appears in collections:Publikationen Life Sciences und Facility Management

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