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https://doi.org/10.21256/zhaw-20120| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS |
| Authors: | Rothen, Julian Sapugahawatte, Dulmini Nanayakkara Li, Carmen Lo, Norman Vogel, Guido Foucault, Frédéric Pflüger, Valentin Pothier, Joël Blom, Jochen Daubenberger, Claudia Ip, Margaret |
| et. al: | No |
| DOI: | 10.1038/s41598-020-65707-5 10.21256/zhaw-20120 |
| Published in: | Scientific Reports |
| Volume(Issue): | 10 |
| Issue: | 8788 |
| Issue Date: | 29-May-2020 |
| Publisher / Ed. Institution: | Nature Publishing Group |
| ISSN: | 2045-2322 |
| Language: | English |
| Subjects: | Medical research; Microbiology |
| Subject (DDC): | 570: Biology |
| Abstract: | Streptococcus agalactiae (Group B Streptococcus, GBS), is a frequent human colonizer and a leading cause of neonatal meningitis as well as an emerging pathogen in non-pregnant adults. GBS possesses a broad animal host spectrum, and recent studies proved atypical GBS genotypes can cause human invasive diseases through animal sources as food-borne zoonotic infections. We applied a MALDI-TOF MS typing method, based on molecular weight variations of predefined 28 ribosomal subunit proteins (rsp) to classify GBS strains of varying serotypes into major phylogenetic lineages. A total of 249 GBS isolates of representative and varying capsular serotypes from patients and animal food sources (fish and pig) collected during 2016-2018 in Hong Kong were analysed. Over 84% (143/171) noninvasive carriage GBS strains from patients were readily typed into 5 globally dominant rsp-profiles. Among GBS strains from food animals, over 90% (57/63) of fish and 13% (2/15) of pig GBS matched with existing rsp-profiles, while the remainder were classified into two novel rsp-profiles and we failed to assign a fish strain into any cluster. MALDI-TOF MS allowed for high-throughput screening and simultaneous detection of novel, so far not well described GBS genotypes. The method shown here is rapid, simple, readily transferable and adapted for use in a diagnostic microbiology laboratory with potential for the surveillance of emerging GBS genotypes with zoonotic potential. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/20120 |
| Fulltext version: | Published version |
| License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Natural Resource Sciences (IUNR) |
| Published as part of the ZHAW project: | MALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approaches |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2020_Rothen_etal_Typing-method-for-Streptococcus-agalactiae-Scientific-Reports.pdf | 1.51 MB | Adobe PDF |  View/Open |
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