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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-20120
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dc.contributor.authorRothen, Julian-
dc.contributor.authorSapugahawatte, Dulmini Nanayakkara-
dc.contributor.authorLi, Carmen-
dc.contributor.authorLo, Norman-
dc.contributor.authorVogel, Guido-
dc.contributor.authorFoucault, Frédéric-
dc.contributor.authorPflüger, Valentin-
dc.contributor.authorPothier, Joël-
dc.contributor.authorBlom, Jochen-
dc.contributor.authorDaubenberger, Claudia-
dc.contributor.authorIp, Margaret-
dc.date.accessioned2020-06-08T07:57:07Z-
dc.date.available2020-06-08T07:57:07Z-
dc.date.issued2020-05-29-
dc.identifier.issn2045-2322de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/20120-
dc.description.abstractStreptococcus agalactiae (Group B Streptococcus, GBS), is a frequent human colonizer and a leading cause of neonatal meningitis as well as an emerging pathogen in non-pregnant adults. GBS possesses a broad animal host spectrum, and recent studies proved atypical GBS genotypes can cause human invasive diseases through animal sources as food-borne zoonotic infections. We applied a MALDI-TOF MS typing method, based on molecular weight variations of predefined 28 ribosomal subunit proteins (rsp) to classify GBS strains of varying serotypes into major phylogenetic lineages. A total of 249 GBS isolates of representative and varying capsular serotypes from patients and animal food sources (fish and pig) collected during 2016-2018 in Hong Kong were analysed. Over 84% (143/171) noninvasive carriage GBS strains from patients were readily typed into 5 globally dominant rsp-profiles. Among GBS strains from food animals, over 90% (57/63) of fish and 13% (2/15) of pig GBS matched with existing rsp-profiles, while the remainder were classified into two novel rsp-profiles and we failed to assign a fish strain into any cluster. MALDI-TOF MS allowed for high-throughput screening and simultaneous detection of novel, so far not well described GBS genotypes. The method shown here is rapid, simple, readily transferable and adapted for use in a diagnostic microbiology laboratory with potential for the surveillance of emerging GBS genotypes with zoonotic potential.de_CH
dc.language.isoende_CH
dc.publisherNature Publishing Groupde_CH
dc.relation.ispartofScientific Reportsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectMedical researchde_CH
dc.subjectMicrobiologyde_CH
dc.subject.ddc570: Biologiede_CH
dc.titleA simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MSde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
dc.identifier.doi10.1038/s41598-020-65707-5de_CH
dc.identifier.doi10.21256/zhaw-20120-
dc.identifier.pmid32472028de_CH
zhaw.funding.euNode_CH
zhaw.issue8788de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume10de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedUmweltgenomikde_CH
zhaw.webfeedHealth Research Hub (LSFM)de_CH
zhaw.funding.zhawMALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approachesde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Rothen, J., Sapugahawatte, D. N., Li, C., Lo, N., Vogel, G., Foucault, F., Pflüger, V., Pothier, J., Blom, J., Daubenberger, C., & Ip, M. (2020). A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS. Scientific Reports, 10(8788). https://doi.org/10.1038/s41598-020-65707-5
Rothen, J. et al. (2020) ‘A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS’, Scientific Reports, 10(8788). Available at: https://doi.org/10.1038/s41598-020-65707-5.
J. Rothen et al., “A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS,” Scientific Reports, vol. 10, no. 8788, May 2020, doi: 10.1038/s41598-020-65707-5.
ROTHEN, Julian, Dulmini Nanayakkara SAPUGAHAWATTE, Carmen LI, Norman LO, Guido VOGEL, Frédéric FOUCAULT, Valentin PFLÜGER, Joël POTHIER, Jochen BLOM, Claudia DAUBENBERGER und Margaret IP, 2020. A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS. Scientific Reports. 29 Mai 2020. Bd. 10, Nr. 8788. DOI 10.1038/s41598-020-65707-5
Rothen, Julian, Dulmini Nanayakkara Sapugahawatte, Carmen Li, Norman Lo, Guido Vogel, Frédéric Foucault, Valentin Pflüger, et al. 2020. “A Simple, Rapid Typing Method for Streptococcus Agalactiae Based on Ribosomal Subunit Proteins by MALDI-TOF MS.” Scientific Reports 10 (8788). https://doi.org/10.1038/s41598-020-65707-5.
Rothen, Julian, et al. “A Simple, Rapid Typing Method for Streptococcus Agalactiae Based on Ribosomal Subunit Proteins by MALDI-TOF MS.” Scientific Reports, vol. 10, no. 8788, May 2020, https://doi.org/10.1038/s41598-020-65707-5.


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