In-Silico UHPLC method optimization for aglycones in the herbal laxatives aloe barbadensis Mill., Cassia angustifolia Vahl Pods, Rhamnus frangula L. Bark, Rhamnus purshianus DC. Bark, and Rheum palmatum L. Roots

Meier, Nadja; Meier, Beat; Peter, Samuel; Wolfram, Evelyn

doi:10.21256/zhaw-3479

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-3479

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	In-Silico UHPLC method optimization for aglycones in the herbal laxatives aloe barbadensis Mill., Cassia angustifolia Vahl Pods, Rhamnus frangula L. Bark, Rhamnus purshianus DC. Bark, and Rheum palmatum L. Roots
Authors:	Meier, Nadja Meier, Beat Peter, Samuel Wolfram, Evelyn
DOI:	10.21256/zhaw-3479 10.3390/molecules22111838
Published in:	Molecules
Volume(Issue):	22
Issue:	11
Issue Date:	27-Oct-2017
Publisher / Ed. Institution:	MDPI
ISSN:	1420-3049 1433-1373
Language:	English
Subjects:	Aloe-emodin; Anthraquinone aglycones; Chromatographic modelling; Chrysophanol; Design of experiment (DOE); Emodin; Herbal laxatives; Physcion; Rhein; Ultra high performance liquid chromatography (UHPLC)
Subject (DDC):	615: Pharmacology and therapeutics 660.6: Biotechnology
Abstract:	For the European Pharmacopoeia (Ph. Eur.) herbal monograph draft of Cassia angustifolia Vahl. and Cassia senna L. leaves and pods, a safety limitation of aloe-emodin and rhein was proposed, due to toxicological concerns. A quantitative, analytical method of the anthraquinone aglycones in all Ph. Eur. monographed herbal laxatives is of interest. A rational method development for the aglycones aloe-emodin, rhein, emodin, chrysophanol, and physcion in five herbal drugs was realized by using 3D chromatographic modelling (temperature, solvent, and gradient time) and design of experiment (DOE) software (DryLab® 4). A methodical approach suitable for the challenging peak tracking in the chromatograms of the herbal drugs in dependence on the changes in the chromatographic conditions is described by using a combination of mass spectroscopy (MS) data (UHPLC-QDa), UV/Vis-spectra, and peak areas. The model results indicate a low robust range and showed that with the selected chromatographic system, small interferences could not be averted. The separation achieved shows a pure UV/Vis spectrum for all aglycones except for chrysophanol in Aloe barbadensis and emodin in Cassia angustifolia fruit. A gradient with the best resolution of the aglycones in all five drugs is proposed, and its suitability demonstrated for the quantification of aglycones in these herbal drugs.
URI:	https://digitalcollection.zhaw.ch/handle/11475/1970
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Meier, N., Meier, B., Peter, S., & Wolfram, E. (2017). In-Silico UHPLC method optimization for aglycones in the herbal laxatives aloe barbadensis Mill., Cassia angustifolia Vahl Pods, Rhamnus frangula L. Bark, Rhamnus purshianus DC. Bark, and Rheum palmatum L. Roots. Molecules, 22(11). https://doi.org/10.21256/zhaw-3479

Meier, N. et al. (2017) ‘In-Silico UHPLC method optimization for aglycones in the herbal laxatives aloe barbadensis Mill., Cassia angustifolia Vahl Pods, Rhamnus frangula L. Bark, Rhamnus purshianus DC. Bark, and Rheum palmatum L. Roots’, Molecules, 22(11). Available at: https://doi.org/10.21256/zhaw-3479.

N. Meier, B. Meier, S. Peter, and E. Wolfram, “In-Silico UHPLC method optimization for aglycones in the herbal laxatives aloe barbadensis Mill., Cassia angustifolia Vahl Pods, Rhamnus frangula L. Bark, Rhamnus purshianus DC. Bark, and Rheum palmatum L. Roots,” Molecules, vol. 22, no. 11, Oct. 2017, doi: 10.21256/zhaw-3479.

MEIER, Nadja, Beat MEIER, Samuel PETER und Evelyn WOLFRAM, 2017. In-Silico UHPLC method optimization for aglycones in the herbal laxatives aloe barbadensis Mill., Cassia angustifolia Vahl Pods, Rhamnus frangula L. Bark, Rhamnus purshianus DC. Bark, and Rheum palmatum L. Roots. Molecules. 27 Oktober 2017. Bd. 22, Nr. 11. DOI 10.21256/zhaw-3479

Meier, Nadja, Beat Meier, Samuel Peter, and Evelyn Wolfram. 2017. “In-Silico UHPLC Method Optimization for Aglycones in the Herbal Laxatives Aloe Barbadensis Mill., Cassia Angustifolia Vahl Pods, Rhamnus Frangula L. Bark, Rhamnus Purshianus DC. Bark, and Rheum Palmatum L. Roots.” Molecules 22 (11). https://doi.org/10.21256/zhaw-3479.

Meier, Nadja, et al. “In-Silico UHPLC Method Optimization for Aglycones in the Herbal Laxatives Aloe Barbadensis Mill., Cassia Angustifolia Vahl Pods, Rhamnus Frangula L. Bark, Rhamnus Purshianus DC. Bark, and Rheum Palmatum L. Roots.” Molecules, vol. 22, no. 11, Oct. 2017, https://doi.org/10.21256/zhaw-3479.