Title: Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots
Authors : Trifan, Adriana
Opitz, Sebastian
Josuran, Roland
Grubelnik, Andreas
Esslinger, Nils
Peter, Samuel
Bräm, Sarah
Meier, Nadja
Wolfram, Evelyn
Published in : Food and Chemical Toxicology
Volume(Issue) : 112
Pages : 178
Pages to: 187
Issue Date: 27-Dec-2017
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Antioxidant activity; Phenolic compound; Pyrrolizidine alkaloid; Salvianolic acid; Symphytum officinale L.
Subject (DDC) : 572: Biochemistry
580: Plants (Botany)
615: Pharmacology and therapeutics
Abstract: Comfrey root preparations are used for the external treatment of joint distortions and myalgia, due to its analgesic and anti-inflammatory properties. Up to date, key activity-determining constituents of comfrey root extracts have not been completely elucidated. Therefore, we applied different approaches to further characterize a comfrey root extract (65% ethanol). The phenolic profile of comfrey root sample was characterized by HPLC-DAD-QTOF-MS/MS. Rosmarinic acid was identified as main phenolic constituent (7.55 mg/g extract). Moreover, trimers and tetramers of caffeic acid (isomers of salvianolic acid A, B and C) were identified and quantified for the first time in comfrey root. In addition, pyrrolizidine alkaloids were evaluated by HPLC-QQQ-MS/MS and acetylintermedine, acetyllycopsamine and their N-oxides were determined as major pyrrolizidine alkaloids in the comfrey root sample. Lastly, the antioxidant activity was determined using four assays: DPPH and ABTS radicals scavenging assays, reducing power assay and 15-lipoxygenase inhibition assay. Comfrey root extract exhibited significant antioxidant activities when compared to known antioxidants. Thus, comfrey root is an important source of phenolic compounds endowed with antioxidant activity which may contribute to the overall bioactivity of Symphytum preparations.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1016/j.fct.2017.12.051
ISSN: 0278-6915
URI: https://digitalcollection.zhaw.ch/handle/11475/1958
Appears in Collections:Publikationen Life Sciences und Facility Management

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