Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-19075
Title: Bradyrhizobium ivorense sp. nov. as a potential local bioinoculant for Cajanus cajan cultures in Côte d’Ivoire
Authors : Fossou, Romain K.
Pothier, Joël F.
Zézé, Adolphe
Perret, Xavier
et. al : No
Published in : International Journal of Systematic and Evolutionary Microbiology
Publisher / Ed. Institution : Microbiology Society
Issue Date: 2-Jan-2020
License (according to publishing contract) : CC BY-NC 4.0: Attribution - Non commercial 4.0 International
Type of review: Peer review (publication)
Language : English
Subjects : Nitrogen fixation; Legume; Root nodule; Pigeonpea; Africa
Subject (DDC) : 570: Biology
Abstract: For many smallholder farmers of Sub-Saharan Africa, pigeonpea (Cajanus cajan) is an important crop to make ends meet. To ascertain the taxonomic status of pigeonpea isolates of Côte d’Ivoire previously identified as bradyrhizobia, a polyphasic approach was applied to strains CI-1BT, CI-14A, CI-19D and CI-41S. Phylogeny of 16S ribosomal RNA (rRNA) genes placed these nodule isolates in a separate lineage from current species of the B. elkanii super clade. In phylogenetic analyses of single and concatenated partial dnaK, glnII, gyrB, recA and rpoB sequences, the C. cajan isolates again formed a separate lineage, with strain CI-1BT sharing the highest sequence similarity (95.2 %) with B. tropiciagri SEMIA 6148T. Comparative genomic analyses corroborated the novel species status, with 86 % ANIb and 89 % ANIm as the highest average nucleotide identity (ANI) values with B. elkanii USDA 76T. Although CI-1BT, CI-14A, CI-19D and CI-41S shared similar phenotypic and metabolic properties, growth of CI-41S was slower in/on various media. Symbiotic efficacy varied significantly between isolates, with CI-1BT and CI-41S scoring on the C. cajan ‘Light-Brown’ landrace as the most and least proficient bacteria, respectively. Also proficient on Vigna radiata (mung bean), Vigna unguiculata (cowpea, niébé) and additional C. cajan cultivars, CI-1BT represents a potential bioinoculant adapted to local soil conditions and capable of fostering the growth of diverse legume crops in Côte d'Ivoire. Given the data presented here, we propose the 19 C. cajan isolates to belong to a novel species called Bradyrhizobium ivorense sp. nov., with CI-1BT (=CCOS 1862T=CCMM B1296T) as a type strain.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Article in scientific journal
DOI : 10.1099/ijsem.0.003931
10.21256/zhaw-19075
ISSN: 1466-5026
1466-5034
URI: https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003931
https://digitalcollection.zhaw.ch/handle/11475/19075
Published as part of the ZHAW project : MinION2FixID: fast and reliable legume root nodule bacteria identification using nanopore sequencing technology
MALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approaches
Appears in Collections:Publikationen Life Sciences und Facility Management

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