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https://doi.org/10.21256/zhaw-1520| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | The histidine-reversible antibiotic herbicolin O produced by Pantoea vagans C9-1 is pantocin A |
| Authors: | Ishimaru, Carol Lansdell, Teresa A. Clardy, Jon Duffy, Brion Smits, Theo H.M. |
| DOI: | 10.4454/jpp.v99i0.3910 10.21256/zhaw-1520 |
| Published in: | Journal of Plant Pathology |
| Volume(Issue): | 99 |
| Issue: | Special issue |
| Page(s): | 91 |
| Pages to: | 97 |
| Issue Date: | Nov-2017 |
| Publisher / Ed. Institution: | Edizioni ETS |
| ISSN: | 2239-7264 |
| Language: | English |
| Subjects: | Fire blight; Biocontrol; Herbicolin O; Peptide antibiotic |
| Subject (DDC): | 570: Biology 630: Agriculture |
| Abstract: | Pantoea vagans C9-1 is one of the most effective and reliable biocontrol agents against fire blight, and has been commercialized as Blight Ban C9-1. Production of multiple antibiotics contributes to its antagonism of Erwinia amylovora. Here we describe the genetics, chemical isolation and structure of herbicolin O, the histidine-reversible antibiotic produced by P. vagans C9-1. Mutational analyses indicated that biosynthesis of herbicolin O depends on paaAB and a sequence encoding the peptide precursor of pantocin A. The paaABC gene cluster encoding biosynthesis and autoresistance was located within a 28-kb chromosomal genomic island of the complete genome sequence of P. vagans C9-1. The cluster was cloned and expressed in E. coli and purified antibiotic was isolated using improved methods for small peptides. The 1H NMR spectra of the C9-1 antibiotic closely resembled those of pantocin A produced by P. agglomerans Eh318. Detailed analysis of the proton spin systems showed that the chemical shift values and coupling constants of the protons in C9-1 herbicolin O correspond exactly to those of pantocin A. Based on these genetic and chemical analyses, herbicolin O and pantocin A are confirmed to be the same antibiotic. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/1741 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Natural Resource Sciences (IUNR) |
| Published as part of the ZHAW project: | Genomik und Transkriptomik von Erwinia und Pantoea |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 57_Ishimaru_JPlantPathol2017.pdf | 315.8 kB | Adobe PDF |  View/Open |
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Ishimaru, C., Lansdell, T. A., Clardy, J., Duffy, B., & Smits, T. H. M. (2017). The histidine-reversible antibiotic herbicolin O produced by Pantoea vagans C9-1 is pantocin A. Journal of Plant Pathology, 99(Special issue), 91–97. https://doi.org/10.4454/jpp.v99i0.3910
Ishimaru, C. et al. (2017) ‘The histidine-reversible antibiotic herbicolin O produced by Pantoea vagans C9-1 is pantocin A’, Journal of Plant Pathology, 99(Special issue), pp. 91–97. Available at: https://doi.org/10.4454/jpp.v99i0.3910.
C. Ishimaru, T. A. Lansdell, J. Clardy, B. Duffy, and T. H. M. Smits, “The histidine-reversible antibiotic herbicolin O produced by Pantoea vagans C9-1 is pantocin A,” Journal of Plant Pathology, vol. 99, no. Special issue, pp. 91–97, Nov. 2017, doi: 10.4454/jpp.v99i0.3910.
ISHIMARU, Carol, Teresa A. LANSDELL, Jon CLARDY, Brion DUFFY und Theo H.M. SMITS, 2017. The histidine-reversible antibiotic herbicolin O produced by Pantoea vagans C9-1 is pantocin A. Journal of Plant Pathology. November 2017. Bd. 99, Nr. Special issue, S. 91–97. DOI 10.4454/jpp.v99i0.3910
Ishimaru, Carol, Teresa A. Lansdell, Jon Clardy, Brion Duffy, and Theo H.M. Smits. 2017. “The Histidine-Reversible Antibiotic Herbicolin O Produced by Pantoea Vagans C9-1 Is Pantocin A.” Journal of Plant Pathology 99 (Special issue): 91–97. https://doi.org/10.4454/jpp.v99i0.3910.
Ishimaru, Carol, et al. “The Histidine-Reversible Antibiotic Herbicolin O Produced by Pantoea Vagans C9-1 Is Pantocin A.” Journal of Plant Pathology, vol. 99, no. Special issue, Nov. 2017, pp. 91–97, https://doi.org/10.4454/jpp.v99i0.3910.
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