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dc.contributor.authorIshimaru, Carol-
dc.contributor.authorLansdell, Teresa A.-
dc.contributor.authorClardy, Jon-
dc.contributor.authorDuffy, Brion-
dc.contributor.authorSmits, Theo H.M.-
dc.description.abstractPantoea vagans C9-1 is one of the most effective and reliable biocontrol agents against fire blight, and has been commercialized as Blight Ban C9-1. Production of multiple antibiotics contributes to its antagonism of Erwinia amylovora. Here we describe the genetics, chemical isolation and structure of herbicolin O, the histidine-reversible antibiotic produced by P. vagans C9-1. Mutational analyses indicated that biosynthesis of herbicolin O depends on paaAB and a sequence encoding the peptide precursor of pantocin A. The paaABC gene cluster encoding biosynthesis and autoresistance was located within a 28-kb chromosomal genomic island of the complete genome sequence of P. vagans C9-1. The cluster was cloned and expressed in E. coli and purified antibiotic was isolated using improved methods for small peptides. The 1H NMR spectra of the C9-1 antibiotic closely resembled those of pantocin A produced by P. agglomerans Eh318. Detailed analysis of the proton spin systems showed that the chemical shift values and coupling constants of the protons in C9-1 herbicolin O correspond exactly to those of pantocin A. Based on these genetic and chemical analyses, herbicolin O and pantocin A are confirmed to be the same antibiotic.de_CH
dc.publisherEdizioni E T Sde_CH
dc.relation.ispartofJournal of Plant Pathologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectFire blightde_CH
dc.subjectHerbicolin Ode_CH
dc.subjectPeptide antibioticde_CH
dc.subject.ddc570: Biologiede_CH
dc.subject.ddc630: Landwirtschaftde_CH
dc.titleThe histidine-reversible antibiotic herbicolin O produced by Pantoea vagans C9-1 is pantocin Ade_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
zhaw.issueSpecial issuede_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.funding.zhawGenomik und Transkriptomik von Erwinia und Pantoeade_CH
Appears in Collections:Publikationen Life Sciences und Facility Management

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