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|Title:||The histidine-reversible antibiotic herbicolin O produced by Pantoea vagans C9-1 is pantocin A|
|Authors :||Ishimaru, Carol|
Lansdell, Teresa A.
Smits, Theo H.M.
|Published in :||Journal of Plant Pathology|
|Issue :||Special issue|
|Publisher / Ed. Institution :||Edizioni E T S|
|Language :||Englisch / English|
|Subjects :||Fire blight; Biocontrol; Herbicolin O; Peptide antibiotic|
|Subject (DDC) :||570: Biologie|
|Abstract:||Pantoea vagans C9-1 is one of the most effective and reliable biocontrol agents against fire blight, and has been commercialized as Blight Ban C9-1. Production of multiple antibiotics contributes to its antagonism of Erwinia amylovora. Here we describe the genetics, chemical isolation and structure of herbicolin O, the histidine-reversible antibiotic produced by P. vagans C9-1. Mutational analyses indicated that biosynthesis of herbicolin O depends on paaAB and a sequence encoding the peptide precursor of pantocin A. The paaABC gene cluster encoding biosynthesis and autoresistance was located within a 28-kb chromosomal genomic island of the complete genome sequence of P. vagans C9-1. The cluster was cloned and expressed in E. coli and purified antibiotic was isolated using improved methods for small peptides. The 1H NMR spectra of the C9-1 antibiotic closely resembled those of pantocin A produced by P. agglomerans Eh318. Detailed analysis of the proton spin systems showed that the chemical shift values and coupling constants of the protons in C9-1 herbicolin O correspond exactly to those of pantocin A. Based on these genetic and chemical analyses, herbicolin O and pantocin A are confirmed to be the same antibiotic.|
|Departement:||Life Sciences und Facility Management|
|Organisational Unit:||Institut für Umwelt und Natürliche Ressourcen (IUNR)|
|Publication type:||Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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