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Title: The histidine-reversible antibiotic herbicolin O produced by Pantoea vagans C9-1 is pantocin A
Authors : Ishimaru, Carol
Lansdell, Teresa A.
Clardy, Jon
Duffy, Brion
Smits, Theo H.M.
Published in : Journal of Plant Pathology
Volume(Issue) : 99
Issue : Special issue
Pages : 91
Pages to: 97
Publisher / Ed. Institution : Edizioni E T S
Issue Date: Nov-2017
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Fire blight; Biocontrol; Herbicolin O; Peptide antibiotic
Subject (DDC) : 570: Biology
630: Agriculture
Abstract: Pantoea vagans C9-1 is one of the most effective and reliable biocontrol agents against fire blight, and has been commercialized as Blight Ban C9-1. Production of multiple antibiotics contributes to its antagonism of Erwinia amylovora. Here we describe the genetics, chemical isolation and structure of herbicolin O, the histidine-reversible antibiotic produced by P. vagans C9-1. Mutational analyses indicated that biosynthesis of herbicolin O depends on paaAB and a sequence encoding the peptide precursor of pantocin A. The paaABC gene cluster encoding biosynthesis and autoresistance was located within a 28-kb chromosomal genomic island of the complete genome sequence of P. vagans C9-1. The cluster was cloned and expressed in E. coli and purified antibiotic was isolated using improved methods for small peptides. The 1H NMR spectra of the C9-1 antibiotic closely resembled those of pantocin A produced by P. agglomerans Eh318. Detailed analysis of the proton spin systems showed that the chemical shift values and coupling constants of the protons in C9-1 herbicolin O correspond exactly to those of pantocin A. Based on these genetic and chemical analyses, herbicolin O and pantocin A are confirmed to be the same antibiotic.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Article in scientific Journal
DOI : 10.4454/jpp.v99i0.3910
ISSN: 2239-7264
Published as part of the ZHAW project : Genomik und Transkriptomik von Erwinia und Pantoea
Appears in Collections:Publikationen Life Sciences und Facility Management

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