Title: Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites
Authors : Bühlmann, Andreas
Dreo, Tanja
Rezzonico, Fabio
Pothier, Joël F.
Smits, Theo H. M.
Ravnikar, Maja
Frey, Jürg E.
Duffy, Brion
Published in : Environmental Microbiology
Volume(Issue) : 16
Issue : 7
Pages : 2112
Pages to: 2125
Publisher / Ed. Institution : Wiley-Blackwell Publishing Ltd.
Issue Date: Jul-2014
Language : Englisch / English
Subject (DDC) : 570: Biologie
Abstract: Erwinia amylovora causes a major disease of pome fruit trees worldwide, and is regulated as a quarantine organism in many countries. While some diversity of isolates has been observed, molecular epidemiology of this bacterium is hindered by a lack of simple molecular typing techniques with sufficiently high resolution. We report a molecular typing system of E. amylovora based on variable number of tandem repeats (VNTR) analysis. Repeats in the E. amylovora genome were identified with comparative genomic tools, and VNTR markers were developed and validated. A Multiple-Locus VNTR Analysis (MLVA) was applied to E. amylovora isolates from bacterial collections representing global and regional distribution of the pathogen. Based on six repeats, MLVA allowed the distinction of 227 haplotypes among a collection of 833 isolates of worldwide origin. Three geographically separated groups were recognized among global isolates using Bayesian clustering methods. Analysis of regional outbreaks confirmed presence of diverse haplotypes but also high representation of certain haplotypes during outbreaks. MLVA analysis is a practical method for epidemiological studies of E. amylovora, identifying previously unresolved population structure within outbreaks. Knowledge of such structure can increase our understanding on how plant diseases emerge and spread over a given geographical region.
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
DOI : 10.1111/1462-2920.12289
ISSN: 1462-2912
URI: https://digitalcollection.zhaw.ch/handle/11475/1711
Appears in Collections:Publikationen Life Sciences und Facility Management

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