Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites

Bühlmann, Andreas; Dreo, Tanja; Rezzonico, Fabio; Pothier, Joël F.; Smits, Theo H. M.; Ravnikar, Maja; Frey, Jürg E.; Duffy, Brion

doi:10.1111/1462-2920.12289

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DC Field	Value	Language
dc.contributor.author	Bühlmann, Andreas	-
dc.contributor.author	Dreo, Tanja	-
dc.contributor.author	Rezzonico, Fabio	-
dc.contributor.author	Pothier, Joël F.	-
dc.contributor.author	Smits, Theo H. M.	-
dc.contributor.author	Ravnikar, Maja	-
dc.contributor.author	Frey, Jürg E.	-
dc.contributor.author	Duffy, Brion	-
dc.date.accessioned	2017-12-05T14:55:22Z	-
dc.date.available	2017-12-05T14:55:22Z	-
dc.date.issued	2014-07	-
dc.identifier.issn	1462-2912	de_CH
dc.identifier.issn	1462-2920	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/1711	-
dc.description.abstract	Erwinia amylovora causes a major disease of pome fruit trees worldwide, and is regulated as a quarantine organism in many countries. While some diversity of isolates has been observed, molecular epidemiology of this bacterium is hindered by a lack of simple molecular typing techniques with sufficiently high resolution. We report a molecular typing system of E. amylovora based on variable number of tandem repeats (VNTR) analysis. Repeats in the E. amylovora genome were identified with comparative genomic tools, and VNTR markers were developed and validated. A Multiple-Locus VNTR Analysis (MLVA) was applied to E. amylovora isolates from bacterial collections representing global and regional distribution of the pathogen. Based on six repeats, MLVA allowed the distinction of 227 haplotypes among a collection of 833 isolates of worldwide origin. Three geographically separated groups were recognized among global isolates using Bayesian clustering methods. Analysis of regional outbreaks confirmed presence of diverse haplotypes but also high representation of certain haplotypes during outbreaks. MLVA analysis is a practical method for epidemiological studies of E. amylovora, identifying previously unresolved population structure within outbreaks. Knowledge of such structure can increase our understanding on how plant diseases emerge and spread over a given geographical region.	de_CH
dc.language.iso	en	de_CH
dc.publisher	Wiley	de_CH
dc.relation.ispartof	Environmental Microbiology	de_CH
dc.rights	Licence according to publishing contract	de_CH
dc.subject.ddc	570: Biologie	de_CH
dc.title	Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Umwelt und Natürliche Ressourcen (IUNR)	de_CH
dc.identifier.doi	10.1111/1462-2920.12289	de_CH
zhaw.funding.eu	No	de_CH
zhaw.issue	7	de_CH
zhaw.originated.zhaw	Yes	de_CH
zhaw.pages.end	2125	de_CH
zhaw.pages.start	2112	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	16	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.webfeed	Umweltgenomik	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Bühlmann, A., Dreo, T., Rezzonico, F., Pothier, J. F., Smits, T. H. M., Ravnikar, M., Frey, J. E., & Duffy, B. (2014). Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites. Environmental Microbiology, 16(7), 2112–2125. https://doi.org/10.1111/1462-2920.12289

Bühlmann, A. et al. (2014) ‘Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites’, Environmental Microbiology, 16(7), pp. 2112–2125. Available at: https://doi.org/10.1111/1462-2920.12289.

A. Bühlmann et al., “Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites,” Environmental Microbiology, vol. 16, no. 7, pp. 2112–2125, Jul. 2014, doi: 10.1111/1462-2920.12289.

BÜHLMANN, Andreas, Tanja DREO, Fabio REZZONICO, Joël F. POTHIER, Theo H. M. SMITS, Maja RAVNIKAR, Jürg E. FREY und Brion DUFFY, 2014. Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites. Environmental Microbiology. Juli 2014. Bd. 16, Nr. 7, S. 2112–2125. DOI 10.1111/1462-2920.12289

Bühlmann, Andreas, Tanja Dreo, Fabio Rezzonico, Joël F. Pothier, Theo H. M. Smits, Maja Ravnikar, Jürg E. Frey, and Brion Duffy. 2014. “Phylogeography and Population Structure of the Biologically Invasive Phytopathogen Erwinia Amylovora Inferred Using Minisatellites.” Environmental Microbiology 16 (7): 2112–25. https://doi.org/10.1111/1462-2920.12289.

Bühlmann, Andreas, et al. “Phylogeography and Population Structure of the Biologically Invasive Phytopathogen Erwinia Amylovora Inferred Using Minisatellites.” Environmental Microbiology, vol. 16, no. 7, July 2014, pp. 2112–25, https://doi.org/10.1111/1462-2920.12289.