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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Cytotoxic effects of pentachlorophenol (PCP) and its metabolite tetrachlorohydroquinone (TCHQ) on liver cells are modulated by antioxidants
Authors : Pietsch-Schmied, Constanze
Hollender, Juliane
Dorusch, Falk
Burkhardt-Holm, Patricia
DOI : 10.21256/zhaw-3393
Published in : Cell Biology and Toxicology
Volume(Issue) : 30
Issue : 4
Pages : 233
Pages to: 252
Issue Date: 2014
Publisher / Ed. Institution : Springer
ISSN: 1573-6822
Language : English
Subjects : Animal; Antioxidant; Ascorbic acid; Cell line; Cell survival; Fish protein; Hepatocyte; Hydroquinones; Inhibitory concentration 50; Oncorhynchus mykiss; Oxidative stress; Pentachlorophenol; Quercetin; Reactive oxygen specie; Superoxide dismutase; Chemical water pollutant
Subject (DDC) : 571: Physiology and related subjects
Abstract: The worldwide distribution and high bioaccumulation potential of pentachlorophenol (PCP) in aquatic organisms imply a high toxicological impact in aquatic systems. Firstly, our investigations show that, similar to mammalian cell lines, PCP can be metabolized to tetrachlorohydroquinone (TCHQ) in the permanent cell line derived from rainbow trout liver cells (RTL-W1). Moreover, we demonstrate that PCP as well as its metabolite TCHQ is capable of influencing the viability of these cells. Three cell viability assays were performed to assess possible cellular targets of these substances. Thus, the cytotoxicity of the PCP-derivative TCHQ was shown for the first time in a fish cell line. Further investigations revealed the involvement of ROS in the cytotoxicity of PCP and its metabolite TCHQ. The observation of oxidative stress provides a plausible explanation for the increased cytotoxicity at higher concentrations especially for PCP and implies possible mechanisms underlying these observations. In addition, antioxidants such as ascorbic acid and quercetin modulate the detrimental effects of PCP and TCHQ whereby both compounds exacerbate the cytotoxic effects of high PCP and TCHQ concentrations.
Further description : Erworben im Rahmen der Schweizer Nationallizenzen (
Fulltext version : Published version
License (according to publishing contract) : Licence according to publishing contract
Restricted until : 2019-07-05
Departement: Life Sciences and Facility Management
Appears in Collections:Publikationen Life Sciences und Facility Management

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