Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Single-molecule identification by spectrally and time-resolved fluorescence detection
Authors : Prummer, Michael
Hübner, Christian G.
Sick, Beate
Hecht, Bert
Renn, Alois
Wild, Urs P.
DOI : 10.1021/ac991116k
Published in : Analytical Chemistry
Volume(Issue) : 72
Issue : 3
Pages : 443
Pages to: 447
Issue Date: 2000
Publisher / Ed. Institution : American Chemical Society
ISSN: 0003-2700
Language : English
Subjects : Singel molecule; Optics; Bayes statistics; Monte carlo
Subject (DDC) : 540: Chemistry
Abstract: A method to identify single molecules rapidly and with high efficiency based on simple probability considerations is proposed. In principle, any property of a detected photon in a single-molecule fluorescence experiment, e.g., emission wavelength, arrival time after pulsed excitation, and polarization, can be analyzed within the framework of the outlined methodology. Monte Carlo simulations show that less than 500 photons are needed to assign an observed single molecule to one out of four species with a confidence level higher than 99.9%. We show that single dye molecules of four different dyes embedded in a polymer film can be identified with time-correlated singlephoton counting spectrally resolved in two channels.
Fulltext version : Published version
License (according to publishing contract) : Licence according to publishing contract
Departement: School of Engineering
Organisational Unit: Institute of Data Analysis and Process Design (IDP)
Appears in Collections:Publikationen School of Engineering

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