|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Single-molecule identification by spectrally and time-resolved fluorescence detection|
Hübner, Christian G.
Wild, Urs P.
|Published in:||Analytical Chemistry|
|Publisher / Ed. Institution:||American Chemical Society|
|Subjects:||Singel molecule; Optics; Bayes statistics; Monte carlo|
|Subject (DDC):||540: Chemistry|
|Abstract:||A method to identify single molecules rapidly and with high efficiency based on simple probability considerations is proposed. In principle, any property of a detected photon in a single-molecule fluorescence experiment, e.g., emission wavelength, arrival time after pulsed excitation, and polarization, can be analyzed within the framework of the outlined methodology. Monte Carlo simulations show that less than 500 photons are needed to assign an observed single molecule to one out of four species with a confidence level higher than 99.9%. We show that single dye molecules of four different dyes embedded in a polymer film can be identified with time-correlated singlephoton counting spectrally resolved in two channels.|
|Fulltext version:||Published version|
|License (according to publishing contract):||Licence according to publishing contract|
|Departement:||School of Engineering|
|Organisational Unit:||Institute of Data Analysis and Process Design (IDP)|
|Appears in collections:||Publikationen School of Engineering|
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Prummer, M., Hübner, C. G., Sick, B., Hecht, B., Renn, A., & Wild, U. P. (2000). Single-molecule identification by spectrally and time-resolved fluorescence detection. Analytical Chemistry, 72(3), 443–447. https://doi.org/10.1021/ac991116k
Prummer, M. et al. (2000) ‘Single-molecule identification by spectrally and time-resolved fluorescence detection’, Analytical Chemistry, 72(3), pp. 443–447. Available at: https://doi.org/10.1021/ac991116k.
M. Prummer, C. G. Hübner, B. Sick, B. Hecht, A. Renn, and U. P. Wild, “Single-molecule identification by spectrally and time-resolved fluorescence detection,” Analytical Chemistry, vol. 72, no. 3, pp. 443–447, 2000, doi: 10.1021/ac991116k.
Prummer, Michael, et al. “Single-Molecule Identification by Spectrally and Time-Resolved Fluorescence Detection.” Analytical Chemistry, vol. 72, no. 3, 2000, pp. 443–47, https://doi.org/10.1021/ac991116k.
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