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dc.contributor.authorPrummer, Michael-
dc.contributor.authorHübner, Christian G.-
dc.contributor.authorSick, Beate-
dc.contributor.authorHecht, Bert-
dc.contributor.authorRenn, Alois-
dc.contributor.authorWild, Urs P.-
dc.date.accessioned2018-12-20T09:14:31Z-
dc.date.available2018-12-20T09:14:31Z-
dc.date.issued2000-
dc.identifier.issn0003-2700de_CH
dc.identifier.issn1520-6882de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/14040-
dc.description.abstractA method to identify single molecules rapidly and with high efficiency based on simple probability considerations is proposed. In principle, any property of a detected photon in a single-molecule fluorescence experiment, e.g., emission wavelength, arrival time after pulsed excitation, and polarization, can be analyzed within the framework of the outlined methodology. Monte Carlo simulations show that less than 500 photons are needed to assign an observed single molecule to one out of four species with a confidence level higher than 99.9%. We show that single dye molecules of four different dyes embedded in a polymer film can be identified with time-correlated singlephoton counting spectrally resolved in two channels.de_CH
dc.language.isoende_CH
dc.publisherAmerican Chemical Societyde_CH
dc.relation.ispartofAnalytical Chemistryde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectSingel moleculede_CH
dc.subjectOpticsde_CH
dc.subjectBayes statisticsde_CH
dc.subjectMonte carlode_CH
dc.subject.ddc540: Chemiede_CH
dc.titleSingle-molecule identification by spectrally and time-resolved fluorescence detectionde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
zhaw.organisationalunitInstitut für Datenanalyse und Prozessdesign (IDP)de_CH
dc.identifier.doi10.1021/ac991116kde_CH
zhaw.funding.euNode_CH
zhaw.issue3de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end447de_CH
zhaw.pages.start443de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume72de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
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Prummer, M., Hübner, C. G., Sick, B., Hecht, B., Renn, A., & Wild, U. P. (2000). Single-molecule identification by spectrally and time-resolved fluorescence detection. Analytical Chemistry, 72(3), 443–447. https://doi.org/10.1021/ac991116k
Prummer, M. et al. (2000) ‘Single-molecule identification by spectrally and time-resolved fluorescence detection’, Analytical Chemistry, 72(3), pp. 443–447. Available at: https://doi.org/10.1021/ac991116k.
M. Prummer, C. G. Hübner, B. Sick, B. Hecht, A. Renn, and U. P. Wild, “Single-molecule identification by spectrally and time-resolved fluorescence detection,” Analytical Chemistry, vol. 72, no. 3, pp. 443–447, 2000, doi: 10.1021/ac991116k.
PRUMMER, Michael, Christian G. HÜBNER, Beate SICK, Bert HECHT, Alois RENN und Urs P. WILD, 2000. Single-molecule identification by spectrally and time-resolved fluorescence detection. Analytical Chemistry. 2000. Bd. 72, Nr. 3, S. 443–447. DOI 10.1021/ac991116k
Prummer, Michael, Christian G. Hübner, Beate Sick, Bert Hecht, Alois Renn, and Urs P. Wild. 2000. “Single-Molecule Identification by Spectrally and Time-Resolved Fluorescence Detection.” Analytical Chemistry 72 (3): 443–47. https://doi.org/10.1021/ac991116k.
Prummer, Michael, et al. “Single-Molecule Identification by Spectrally and Time-Resolved Fluorescence Detection.” Analytical Chemistry, vol. 72, no. 3, 2000, pp. 443–47, https://doi.org/10.1021/ac991116k.


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