Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4886
Title: Sample preparation of multiple steroids for quantitative determination by LC-MS/MS : comparison of two extraction procedures
Authors : Sebregondi, Patrizia
Zaborosch, Christiane
van Staveren, Dave
Geyer, Roland
Svoboda, Michal
Conference details: MSACL 2015 US Congress, San Diego, USA, March 28 - April 1 2015
Publisher / Ed. Institution : Tecan Trading
Issue Date: 2015
License (according to publishing contract) : Not specified
Type of review: Not specified
Language : English
Subject (DDC) : 572: Biochemistry
Abstract: Testosterone, androstenedione and 17-hydroxyprogesterone are powerful steroids which play a critical role in development and reproductive processes in men and women. The quantitative determination of serum steroids is mandatory in the investigation of suspected disorder of excessive and insufficient steroid production in adults and delayed puberty in adolescents. While immunoassays are the general analytical method, for accurate and reproducible quantification of such analytes liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is gaining importance. Advantages are its unparalleled specificity and sensitivity combined with the very practical potential for multi-analyte quantification. Prior to any analysis by LC-MS/MS a suitable sample clean-up is essential to efficiently remove matrix components such as proteins, lipids, carbohydrates and salt which may interfere with the analytical procedure. The Tecan AC Extraction PlateTM is an ingenious novel consumable: a 96-position, deep-well microplate featuring TICETM (Tecan Immobilized Coating Extraction) technology (Figure 1). The inner surface of each well is coated with a specific volume of sorptive material to approximately half the well’s height. The TICE coating acts as the extractant, eliminating the need for protein precipitation, liquid separation steps or the generation of emulsions. It is the basis for a simple and automatable approach to the preparation of biological samples without the need for offline operations. In parallel an automation for SLE+ plates from Biotage was set up on the same Tecan liquid handling system for comparison.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Conference Poster
DOI : 10.21256/zhaw-4886
URI: https://digitalcollection.zhaw.ch/handle/11475/13178
Published as part of the ZHAW project : Innovative Extraktionstechnologie zur automatisierten Probenvorbereitung für Massenspektrometrie-Applikationen im integrierten Labor
Appears in Collections:Publikationen Life Sciences und Facility Management

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