Electro-spinning of pure collagen fibres : just an expensive way to make gelatin?

Zeugolis, Dimitrios I.; Khew, Shih T.; Yew, Elijah S.Y.; Ekaputra, Andrew K.; Tong, Yen W.; Yung, Lin-Yue L.; Hutmacher, Dietmar W.; Sheppard, Colin; Raghunath, Michael

doi:10.1016/j.biomaterials.2008.02.009

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Electro-spinning of pure collagen fibres : just an expensive way to make gelatin?
Authors:	Zeugolis, Dimitrios I. Khew, Shih T. Yew, Elijah S.Y. Ekaputra, Andrew K. Tong, Yen W. Yung, Lin-Yue L. Hutmacher, Dietmar W. Sheppard, Colin Raghunath, Michael
DOI:	10.1016/j.biomaterials.2008.02.009
Published in:	Biomaterials
Volume(Issue):	29
Issue:	15
Page(s):	2293
Pages to:	2305
Issue Date:	2008
Publisher / Ed. Institution:	Elsevier
ISSN:	0142-9612
Language:	English
Subject (DDC):	572: Biochemistry
Abstract:	Scaffolds manufactured from biological materials promise better clinical functionality, providing that characteristic features are preserved. Collagen, a prominent biopolymer, is used extensively for tissue engineering applications, because its signature biological and physico-chemical properties are retained in in vitro preparations. We show here for the first time that the very properties that have established collagen as the leading natural biomaterial are lost when it is electro-spun into nano-fibres out of fluoroalcohols such as 1,1,1,3,3,3-hexafluoro-2-propanol or 2,2,2-trifluoroethanol. We further identify the use of fluoroalcohols as the major culprit in the process. The resultant nano-scaffolds lack the unique ultra-structural axial periodicity that confirms quarter-staggered supramolecular assemblies and the capacity to generate second harmonic signals, representing the typical crystalline triple-helical structure. They were also characterised by low denaturation temperatures, similar to those obtained from gelatin preparations (p > 0.05). Likewise, circular dichroism spectra revealed extensive denaturation of the electro-spun collagen. Using pepsin digestion in combination with quantitative SDS-PAGE, we corroborate great losses of up to 99% of triple-helical collagen. In conclusion, electro-spinning of collagen out of fluoroalcohols effectively denatures this biopolymer, and thus appears to defeat its purpose, namely to create biomimetic scaffolds emulating the collagen structure and function of the extracellular matrix.
URI:	https://digitalcollection.zhaw.ch/handle/11475/12207
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Zeugolis, D. I., Khew, S. T., Yew, E. S. Y., Ekaputra, A. K., Tong, Y. W., Yung, L.-Y. L., Hutmacher, D. W., Sheppard, C., & Raghunath, M. (2008). Electro-spinning of pure collagen fibres : just an expensive way to make gelatin? Biomaterials, 29(15), 2293–2305. https://doi.org/10.1016/j.biomaterials.2008.02.009

Zeugolis, D.I. et al. (2008) ‘Electro-spinning of pure collagen fibres : just an expensive way to make gelatin?’, Biomaterials, 29(15), pp. 2293–2305. Available at: https://doi.org/10.1016/j.biomaterials.2008.02.009.

D. I. Zeugolis et al., “Electro-spinning of pure collagen fibres : just an expensive way to make gelatin?,” Biomaterials, vol. 29, no. 15, pp. 2293–2305, 2008, doi: 10.1016/j.biomaterials.2008.02.009.

ZEUGOLIS, Dimitrios I., Shih T. KHEW, Elijah S.Y. YEW, Andrew K. EKAPUTRA, Yen W. TONG, Lin-Yue L. YUNG, Dietmar W. HUTMACHER, Colin SHEPPARD und Michael RAGHUNATH, 2008. Electro-spinning of pure collagen fibres : just an expensive way to make gelatin? Biomaterials. 2008. Bd. 29, Nr. 15, S. 2293–2305. DOI 10.1016/j.biomaterials.2008.02.009

Zeugolis, Dimitrios I., Shih T. Khew, Elijah S.Y. Yew, Andrew K. Ekaputra, Yen W. Tong, Lin-Yue L. Yung, Dietmar W. Hutmacher, Colin Sheppard, and Michael Raghunath. 2008. “Electro-Spinning of Pure Collagen Fibres : Just an Expensive Way to Make Gelatin?” Biomaterials 29 (15): 2293–2305. https://doi.org/10.1016/j.biomaterials.2008.02.009.

Zeugolis, Dimitrios I., et al. “Electro-Spinning of Pure Collagen Fibres : Just an Expensive Way to Make Gelatin?” Biomaterials, vol. 29, no. 15, 2008, pp. 2293–305, https://doi.org/10.1016/j.biomaterials.2008.02.009.