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dc.contributor.authorZeugolis, Dimitrios I.-
dc.contributor.authorKhew, Shih T.-
dc.contributor.authorYew, Elijah S.Y.-
dc.contributor.authorEkaputra, Andrew K.-
dc.contributor.authorTong, Yen W.-
dc.contributor.authorYung, Lin-Yue L.-
dc.contributor.authorHutmacher, Dietmar W.-
dc.contributor.authorSheppard, Colin-
dc.contributor.authorRaghunath, Michael-
dc.date.accessioned2018-10-26T14:47:58Z-
dc.date.available2018-10-26T14:47:58Z-
dc.date.issued2008-
dc.identifier.issn0142-9612de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12207-
dc.description.abstractScaffolds manufactured from biological materials promise better clinical functionality, providing that characteristic features are preserved. Collagen, a prominent biopolymer, is used extensively for tissue engineering applications, because its signature biological and physico-chemical properties are retained in in vitro preparations. We show here for the first time that the very properties that have established collagen as the leading natural biomaterial are lost when it is electro-spun into nano-fibres out of fluoroalcohols such as 1,1,1,3,3,3-hexafluoro-2-propanol or 2,2,2-trifluoroethanol. We further identify the use of fluoroalcohols as the major culprit in the process. The resultant nano-scaffolds lack the unique ultra-structural axial periodicity that confirms quarter-staggered supramolecular assemblies and the capacity to generate second harmonic signals, representing the typical crystalline triple-helical structure. They were also characterised by low denaturation temperatures, similar to those obtained from gelatin preparations (p > 0.05). Likewise, circular dichroism spectra revealed extensive denaturation of the electro-spun collagen. Using pepsin digestion in combination with quantitative SDS-PAGE, we corroborate great losses of up to 99% of triple-helical collagen. In conclusion, electro-spinning of collagen out of fluoroalcohols effectively denatures this biopolymer, and thus appears to defeat its purpose, namely to create biomimetic scaffolds emulating the collagen structure and function of the extracellular matrix.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofBiomaterialsde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleElectro-spinning of pure collagen fibres : just an expensive way to make gelatin?de_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.biomaterials.2008.02.009de_CH
zhaw.funding.euNode_CH
zhaw.issue15de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end2305de_CH
zhaw.pages.start2293de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume29de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
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Zeugolis, D. I., Khew, S. T., Yew, E. S. Y., Ekaputra, A. K., Tong, Y. W., Yung, L.-Y. L., Hutmacher, D. W., Sheppard, C., & Raghunath, M. (2008). Electro-spinning of pure collagen fibres : just an expensive way to make gelatin? Biomaterials, 29(15), 2293–2305. https://doi.org/10.1016/j.biomaterials.2008.02.009
Zeugolis, D.I. et al. (2008) ‘Electro-spinning of pure collagen fibres : just an expensive way to make gelatin?’, Biomaterials, 29(15), pp. 2293–2305. Available at: https://doi.org/10.1016/j.biomaterials.2008.02.009.
D. I. Zeugolis et al., “Electro-spinning of pure collagen fibres : just an expensive way to make gelatin?,” Biomaterials, vol. 29, no. 15, pp. 2293–2305, 2008, doi: 10.1016/j.biomaterials.2008.02.009.
ZEUGOLIS, Dimitrios I., Shih T. KHEW, Elijah S.Y. YEW, Andrew K. EKAPUTRA, Yen W. TONG, Lin-Yue L. YUNG, Dietmar W. HUTMACHER, Colin SHEPPARD und Michael RAGHUNATH, 2008. Electro-spinning of pure collagen fibres : just an expensive way to make gelatin? Biomaterials. 2008. Bd. 29, Nr. 15, S. 2293–2305. DOI 10.1016/j.biomaterials.2008.02.009
Zeugolis, Dimitrios I., Shih T. Khew, Elijah S.Y. Yew, Andrew K. Ekaputra, Yen W. Tong, Lin-Yue L. Yung, Dietmar W. Hutmacher, Colin Sheppard, and Michael Raghunath. 2008. “Electro-Spinning of Pure Collagen Fibres : Just an Expensive Way to Make Gelatin?” Biomaterials 29 (15): 2293–2305. https://doi.org/10.1016/j.biomaterials.2008.02.009.
Zeugolis, Dimitrios I., et al. “Electro-Spinning of Pure Collagen Fibres : Just an Expensive Way to Make Gelatin?” Biomaterials, vol. 29, no. 15, 2008, pp. 2293–305, https://doi.org/10.1016/j.biomaterials.2008.02.009.


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